EPSTEIN-BARR-VIRUS LATENT MEMBRANE-PROTEIN EXPRESSION IN HODGKIN AND REED-STERNBERG CELLS
EPSTEIN-BARR-VIRUS LATENT MEMBRANE-PROTEIN EXPRESSION IN HODGKIN AND REED-STERNBERG CELLS
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DOI：
10.1073/pnas.88.11.4766
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发表时间：
1991-06-01
影响因子：
11.1
通讯作者：
STEIN, H
中科院分区：
文献类型：
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作者：
HERBST, H;DALLENBACH, F;STEIN, H
对47例霍奇金病患者淋巴结的冷冻切片进行了EB病毒（EBV）编码的潜伏膜蛋白（LMP）、核抗原2和晚期病毒糖蛋白gp 350/250的免疫组织学检查。 一个独特的LMP特异性膜和细胞质染色检测仅在霍奇金和Reed-Sternberg细胞18例（38%），EBV核抗原2和gp 350/250免疫反应性在所有情况下都不存在。 47例病例中有32例（68%）通过PCR检测到EBV特异性DNA序列，所有LMP阳性病例均属于这一类。 我们的研究结果证实了以前的研究建立的EBV基因组在霍奇金和Reed-Sternberg细胞中的存在，通过展示EBV编码的蛋白质在肿瘤细胞群中的表达。 LMP表达的EBV核抗原2的情况下的发现表明，EBV基因的表达模式不同的B-淋巴母细胞样细胞系和伯基特淋巴瘤，而这一发现显示与未分化鼻咽癌中所见的相似性。 由于LMP基因具有转化潜力，我们的研究结果支持EBV在许多霍奇金病病例中的病理病因作用的概念。
Cryostat sections from lymph nodes of 47 Hodgkin disease patients were examined by immunohistology for the Epstein-Barr virus (EBV)-encoded latent membrane protein (LMP), nuclear antigen 2, and late viral glycoprotein gp350/250. A distinct LMP-specific membrane and cytoplasmic staining was detected exclusively in Hodgkin and Reed-Sternberg cells in 18 patients (38%); EBV nuclear antigen 2 and gp350/250 immunoreactivity was absent in all instances. Thirty-two of 47 (68%) cases contained EBV-specific DNA sequences as detected by PCR, all LMP-positive cases being in this category. Our results confirm previous studies establishing the presence of EBV genomes in Hodgkin and Reed-Sternberg cells by demonstrating expression of an EBV-encoded protein in the tumor-cell population. The finding of LMP expression in the absence of EBV nuclear antigen 2 suggests a pattern of EBV gene expression different from that of B-lymphoblastoid cell lines and Burkitt lymphoma, whereas this finding shows similarities with that seen in undifferentiated nasopharyngeal carcinoma. Because the LMP gene has transforming potential, our findings support the concept of a pathoetiological role of EBV in many cases of Hodgkin disease.