Molecularly imprinted CEC sorbents: investigations into polymer preparation and electrolyte composition.
Molecularly imprinted CEC sorbents: investigations into polymer preparation and electrolyte composition.
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DOI：
10.1039/b105104k
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发表时间：
2002-01
期刊：
影响因子：
--
通讯作者：
L. Schweitz;L. Andersson;S. Nilsson
中科院分区：
文献类型：
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作者：
L. Schweitz;L. Andersson;S. Nilsson
研究了毛细管电色谱(CEC)中吸附剂制备工艺和分离参数对超孔分子印迹聚合物(MIP)整体柱选择性和色层效率的影响。采用手性模板，以对映体分离和拆分分别作为印迹选择性和柱效的指标，并用非相关芳香族结构的色谱学对后者进行了研究。聚合物制备因预聚合混合物中的单体组成以及单模板和多模板的使用而有所不同(S)。考察了电解液中有机溶剂和表面活性剂改性剂的种类和含量对分离参数的影响。研究发现，在混合缓冲液中，丙酮和乙腈对模板对映体和结构类似物均有较好的拆分效果，但拆分程度受电解液含量的影响较大。考察了十二烷基硫酸钠(十二烷基硫酸钠)、十六烷基三甲基溴化铵(CTAB)和聚氧乙烯山梨醇单月桂酸酯(Tween20)三种表面活性剂作为电解液改进剂的效果。结果表明，十二烷基硫酸钠的加入量减少，CTAB和吐温20的加入增加了对映体的拆分。十二烷基硫酸钠和十六烷基三甲基溴化铵可以用到1 mM浓度，而吐温可以用到90 mM浓度而不会引起基线干扰。发现和证明的效果强烈地表明，在优化MIP-CEC系统时应考虑这些参数。
The influence of the sorbent preparation protocol and separation parameters on the selectivity and chromatographic efficiency of super-porous molecularly imprinted polymer (MIP) monoliths in capillary electrochromatography (CEC) was studied. Chiral templates were employed and enantiomer separation and resolution were used as measures of imprint selectivity and column efficiency, respectively; the latter was in addition studied by chromatography of non-related aromatic structures. The polymer preparation was varied with respect to monomer composition in the pre-polymerisation mixture and also the use of single versus multiple template(s). The separation parameters investigated were type and content of organic solvent and surfactant modifier in the electrolyte. It was found that acetone and acetonitrile in buffer mixtures provided enantiomer separation of enantiomers of the template and also structural analogues; however, the degree of separation was greatly influenced by the content in the electrolyte. Three surfactants, sodium dodecylsulfate (SDS), cetyltrimethylammonium bromide (CTAB) and polyoxyethylene sorbitanmonolaurate (Tween 20), were examined as electrolyte modifiers. It was found that addition of SDS decreased and CTAB and Tween 20 increased the enantiomer separation. SDS and CTAB could be used up to 1 mM concentration whereas Tween could be used up to 90 mM concentration without causing baseline disturbances. The effects found and demonstrated strongly suggest that these parameters are to be considered during optimisation of an MIP-CEC system.