LINKAGE OF THE CALMODULIN AND UBIQUITIN LOCI IN TRYPANOSOMA-CRUZI
LINKAGE OF THE CALMODULIN AND UBIQUITIN LOCI IN TRYPANOSOMA-CRUZI
复制标题
DOI：
10.1093/nar/18.15.4561
复制
发表时间：
1990-08-11
影响因子：
14.9
通讯作者：
SWINDLE, J
中科院分区：
文献类型：
--
作者：
CHUNG, SH;SWINDLE, J
We describe here the organization of the calmodulin genes of Trypanosoma cruzi and their linkage to the ubiquitin gene family. The nucleotide sequence of the CalA2 gene has been determined and is 85% homologous to the protein coding sequence of the calmodulin genes of the African trypanosome, Trypanosoma brucei. The proteins encoded by CalA2 and the T. brucei genes contain a single mismatch out of a total of 149 amino acids. The genome of T. cruzi contains eight calmodulin genes present at two distinct loci (2.8 and 2.65) each of which is linked to downsteam ubiquitin genes. Within each locus two calmodulin gene families have been defined, CalA and CalB. Each calmodulin locus consists of alternating tandem arrays of the CalA and CalB genes. Both 2.8 and 2.65 calmodulin loci and their respective downstream ubiquitin genes share the same DNA coding strand. Transcription of the calmodulin genes in the epimastigote stage of T. cruzi results in the generation of two stable mRNAs of 1.6Kb and 1.1Kb.
