Transgenic overexpression of P450 genes confers deltamethrin resistance in the fall armyworm, Spodoptera frugiperda
Transgenic overexpression of P450 genes confers deltamethrin resistance in the fall armyworm, Spodoptera frugiperda
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DOI：
10.1007/s10340-021-01452-6
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发表时间：
2021-10-29
影响因子：
4.8
通讯作者：
Palli, Subba Reddy
中科院分区：
文献类型：
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作者：
Chen, Xien;Palli, Subba Reddy
秋粘虫 (FAW) 草地贪夜蛾 (Spodoptera frugiperda) 是一种多食性害虫，以许多寄主植物为食，包括一些主要作物，如玉米和水稻。这种害虫还对许多杀虫剂产生了抗药性。最近的基因组和转录组测序工作在秋粘虫中发现了 117-425 个 P450 基因，但它们在解毒植物毒素和杀虫剂方面的功能很大程度上未知。这项研究发现，在饲喂溴氰菊酯的一龄秋粘虫幼虫中，P450 基因 SfCYP321A8 表达上调。生产过表达 SfCYP321A8 的转基因 FAW 以研究其在溴氰菊酯抗性中的功能。还产生了转基因 FAW，其表达编码已知可代谢赤拟谷盗中溴氰菊酯的 P450 的基因，即 TcCYP6BQ9。 P450基因在转基因幼虫的不同组织中高表达。两种转基因FAW品系的中肠和脂肪体中的P450活性显着高于野生型幼虫。溴氰菊酯生物测定表明，表达SfCYP321A8或TcCYP6BQ9的转基因幼虫的耐受性分别是野生型幼虫的10.3倍或15.3倍。这些研究报告了表达 P450 基因的秋粘虫转基因株系的生产，并表明 SfCYP321A8 有助于秋粘虫对溴氰菊酯的抗性。开发的转化方法可用于秋粘虫和其他鳞翅目害虫的功能基因组学研究。
The fall armyworm (FAW), Spodoptera frugiperda, is a polyphagous insect pest feeding on many host plants, including some major crops such as corn and rice. This pest has also developed resistance to many insecticides. Recent genome and transcriptome sequencing efforts identified 117-425 P450 genes in the FAW, but their function in detoxifying plant toxins and insecticides is largely unknown. This study found that a P450 gene, SfCYP321A8, is upregulated in the first instar FAW larvae fed on deltamethrin. A transgenic FAW overexpressing SfCYP321A8 was produced to investigate its function in deltamethrin resistance. Transgenic FAW expressing the gene coding for a P450 known to metabolize deltamethrin in Tribolium castaneum, TcCYP6BQ9, was also produced. P450 genes are highly expressed in different tissues of transgenic larvae. The P450 activity in the midgut and fat body of both transgenic FAW lines is significantly higher than in wild-type larvae. Deltamethrin bioassays showed that the transgenic larvae expressing SfCYP321A8 or TcCYP6BQ9 are 10.3- or 15.3-fold more tolerant, respectively, than the wild-type larvae. These studies report on the production of FAW transgenic lines expressing P450 genes and show that SfCYP321A8 contributes to deltamethrin resistance in FAW. The transformation methods developed could be used in functional genomics studies in FAW and other lepidopteran pests.