MEMBRANE ASYMMETRY IN EPITHELIA - IS THE TIGHT JUNCTION A BARRIER TO DIFFUSION IN THE PLASMA-MEMBRANE
MEMBRANE ASYMMETRY IN EPITHELIA - IS THE TIGHT JUNCTION A BARRIER TO DIFFUSION IN THE PLASMA-MEMBRANE
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DOI：
10.1038/294718a0
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发表时间：
1981-01-01
期刊：
影响因子：
64.8
通讯作者：
HANDLER, JS
中科院分区：
文献类型：
--
作者：
DRAGSTEN, PR;BLUMENTHAL, R;HANDLER, JS
膜结合[花生和小麦胚芽]凝集素和一些脂质探针不能通过上皮[蟾蜍肾A6细胞，猪LLC-PK 1细胞，狗MDCK细胞]细胞膜的紧密连接区。凝集素在细胞表面上是固定的，但脂质探针在膜中自由扩散。脂质探针通过紧密连接的能力与其翻转至细胞膜双层的内单层的能力相关。
Membrane-bound [peanut and wheat germ] lectins and some lipid probes are incapable of passing through the tight junction region of epithelial [toad kidney A6 cells, pig LLC-PK1 cells, dog MDCK cells] cell membranes. The lectins are immobile on the cell surface, but lipid probes diffuse freely in the membrane. The ability of a lipid probe to pass the tight junction is correlated with its ability to flip-flop to the inner monolayer of the cell membrane bilayer.