Cloning of the IL-1β3 gene and IL-1β4 pseudogene in salmonids uncovers a second type of IL-1β gene in teleost fish
Cloning of the IL-1β3 gene and IL-1β4 pseudogene in salmonids uncovers a second type of IL-1β gene in teleost fish
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DOI：
10.1016/j.dci.2012.07.010
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发表时间：
2012-11-01
影响因子：
2.9
通讯作者：
Wang, Tiehui
中科院分区：
文献类型：
--
作者：
Husain, Mansourah;Bird, Steve;Wang, Tiehui
迄今为止，在鲑鱼中发现了两个密切相关的白细胞介素 1 β 基因（IL-1 β 1 和 IL-β 2）。鳟鱼和鲑鱼 IL-1 beta 3 以及鲑鱼 IL-1 beta 4 假基因的克隆揭示了硬骨鱼物种中存在两种类型的 IL-1 beta 基因。 I 型硬骨鱼 IL-1 beta 基因，包括鲑鱼 IL-1 beta 3，与四足动物具有相似的 6 编码外显子结构。 II 型硬骨鱼 IL-1 β 基因，例如鲑鱼 IL-1 beta 1-2，在其 5' 端缺少一两个编码外显子，并且在该亚组内比在 I 型亚组内具有更高的同一性。这两种类型的 IL-1 β 基因均在鲑形目、鲈形目和贝罗尼形目物种中发现，表明这种分化发生在硬骨鱼进化的早期。鳟鱼 IL-1 beta 3 在卵巢中高表达，表明其在生殖中发挥作用。在鳃、脾和肾中相对较高的组成型表达以及 PAMP、促炎细胞因子和病毒感染的上调表明 IL-1 beta 3 在炎症和宿主防御中也发挥着作用。 (C) 2012 Elsevier Ltd. 保留所有权利。
To date two closely related interleukin-1 beta genes (IL-1 beta 1 and IL-beta 2) have been found in salmonids. The cloning of trout and salmon IL-1 beta 3, and a salmon IL-1 beta 4 pseudogene reveals that two types of IL-1 beta genes exist in teleost species. Type I teleost IL-1 beta genes, including salmonid IL-1 beta 3, share a similar 6 coding exon structure as in tetrapods. Type II teleost IL-1 beta genes, e.g. salmonid IL-1 beta 1-2, lack one or two coding exons at their 5'-end, and share higher identities within this subgroup than within the type I subgroup. Both types of IL-1 beta genes have been found in species of Salmoniformes, Perciformes and Beloniformes, suggesting the divergence occurred early in teleost evolution. Trout IL-1 beta 3 is highly expressed in ovary suggesting a role in reproduction. A relatively high constitutive expression in gills, spleen and kidney and the up-regulation by PAMPs, proinflammatory cytokines and viral infection suggests IL-1 beta 3 also has a role in inflammation and host defence. (C) 2012 Elsevier Ltd. All rights reserved.