Apelin is a novel angiogenic factor in retinal endothelial cells
Apelin is a novel angiogenic factor in retinal endothelial cells
复制标题
DOI：
10.1016/j.bbrc.2004.10.042
复制
发表时间：
2004-12-10
影响因子：
3.1
通讯作者：
Baba, A
中科院分区：
文献类型：
--
作者：
Kasai, A;Shintani, N;Baba, A
apelin是孤儿g蛋白偶联受体APJ的内源性配体，近年来在心血管和中枢神经系统中可能具有的功能受到了广泛的关注。我们报道了apelin作为一种新的血管生成因子在视网膜内皮细胞中的新功能。视网膜内皮细胞系RF/6A高度表达apelin和AN转录本，而人脐静脉内皮细胞（HUVECs）仅表达apelin mRNA。根据这些观察，I pM-1 muM浓度下的apelin显著增强了RF/6A细胞的迁移、增殖和毛细血管样管的形成，但对HUVECs没有影响，而VEGF对两种细胞类型的这些参数都有刺激作用。在血管生成的体内Matrigel堵塞试验中，在Matrigel中包含I nM apelin导致清晰的毛细血管样结构，并增加了堵塞中的血红蛋白含量。这是第一次报道显示视网膜内皮细胞中apelin是一种血管生成因子。(C) 2004爱思唯尔公司版权所有。
There has been much focus recently on the possible functions of apelin, an endogenous ligand for the orphan G-protein-coupled receptor APJ, in cardiovascular and central nervous systems. We report a new function of apelin as a novel angiogenic factor in retinal endothelial cells. The retinal endothelial cell line RF/6A highly expressed both apelin and AN transcripts, while human umbilical venous endothelial cells (HUVECs) only expressed apelin mRNA. In accordance with these observations, apelin at concentrations of I pM-1 muM significantly enhanced migration, proliferation, and capillary-like tube formation of RF/6A cells, but not those of HUVECs, whereas VEGF stimulates those parameters of both cell types. In vivo Matrigel plug assay for angiogenesis, the inclusion of I nM apelin in the Matrigel resulted in clear capillary-like formations with an increase of hemoglobin content in the plug. This is the first report showing that apelin is an angiogenic factor in retinal endothelial cells. (C) 2004 Elsevier Inc. All rights reserved.