Characterization and hypoglycemic effect of a neutral polysaccharide extracted from the residue of Codonopsis Pilosula
Characterization and hypoglycemic effect of a neutral polysaccharide extracted from the residue of Codonopsis Pilosula
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党参残渣中提取的中性多糖的表征及其降血糖作用。
DOI：
10.1016/j.carbpol.2018.05.067
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发表时间：
2018-10-01
影响因子：
11.2
通讯作者：
Gao, Xiangdong
中科院分区：
文献类型：
--
作者：
Liu, Wei;Lv, Xing;Gao, Xiangdong
采用乙醇沉淀法和柱层析法从党参残渣中提取纯化了一种中性多糖，命名为CERP1。通过HPAEC-PAD、高效液相色谱、HPSEC-MALLS、FT-IR、核磁共振、透射电子显微镜等手段确定了CERP1的结构。结果表明，CERP1是由阿拉伯糖、葡萄糖和半乳糖组成的杂多糖，其比例为1.00：19.83：6.94。HPSEC-MALLS分析表明，CERP1分子量均一，绝对分子量为4.840×10(3)Da。核磁共振和GC-MS甲基化结果表明，CERP1中的主要键为1-键、1，3-键、1，6-键和1，3，6-键。透射电子显微镜结果表明，CERP1在纯净水和0.05M硫酸钠中粒径均一，分散性好。CERP1可显著改善INS-1细胞的胰岛素分泌。体内实验表明，CERP1对T2 DM小鼠具有明显的降糖作用，能减轻氧化应激，改善脂质代谢，提高糖酵解酶活性，降低肝脏转氨酶活性。本研究为党参残渣的开发利用提供了契机。
A neutral polysaccharide coded as CERP1 was extracted and purified from the residue of Codonopsis pilosula by ethanol precipitation and column chromatography. The structure of CERP1 was determined by HPAEC-PAD, HPLC, HPSEC-MALLS, FT-IR, NMR, and TEM. The results showed that CERP1 was a heteropolysaccharide composed of Arabinose, Glucose, and Galactose in the ratio of 1.00:19.83:6.94. HPSEC-MALLS showed that CERP1 was homogeneous with an absolute molecular weight of 4.840 x 10(3) Da. The NMR results and the GC-MS result of methylation indicated that 1-linked beta-D-glucose, 1,3-linked beta-D-glucose, 1,6-linked beta-D-glucose and 1,3,6-linked beta-D-galactose were the main linkages in CERP1. TEM results indicated that CERP1 had a homogeneous particle size and good dispersibility both in purified water and 0.05 M sodium sulfate. Pretreatment with CERP1 could result in a significant improvement of the insulin secretion of the INS-1 cells. While in vivo assays, CERP1 exhibited a potent hypoglycemic effect on T2DM mice for its ability to relieve oxidative stress, ameliorate lipid metabolism, increase glycolytic enzyme activity as well as decrease liver transaminase activity. This study gives a chance for exploring the residue of Codonopsis pilosula.