The association of calprotectin level in gingival crevicular fluid with gingival index and the activities of collagenase and aspartate aminotransferase in adult periodontitis patients
The association of calprotectin level in gingival crevicular fluid with gingival index and the activities of collagenase and aspartate aminotransferase in adult periodontitis patients
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DOI：
10.1902/jop.2000.71.3.361
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发表时间：
2000-03-01
影响因子：
4.3
通讯作者：
Nagata, T
中科院分区：
文献类型：
--
作者：
Nakamura, T;Kido, J;Nagata, T
背景：钙保护蛋白是白细胞的一种主要细胞质蛋白，存在于健康人的血浆和其他体液中。由于钙保护蛋白浓度在一些炎症性疾病（包括风湿性关节炎）中显著升高，该蛋白被认为是炎症性疾病的标志。最近，我们在人牙石和龈沟液（GCF）中检测到钙保护蛋白，发现牙周炎患者龈沟液中钙保护蛋白浓度明显高于健康人龈沟液。本研究通过研究GCF钙保护蛋白水平与GCF体积、牙龈指数（GI）以及GCF中胶原酶、谷草转氨酶（AST）等生化指标水平的关系，探讨GCF钙保护蛋白水平与牙周炎症的关系。方法：对54例成人牙周炎患者进行牙周袋探探深度大于4mm的90份GCF标本进行检测。测量GCF体积，记录各部位GI。ELISA法测定GCF样品中钙保护蛋白的含量。用相应的检测试剂盒检测胶原酶或AST的活性。结果：钙保护蛋白总量与GCF体积呈极显著相关(r = 0.64， P
Background: Calprotectin, a major cytosol protein of leukocytes, exists in plasma and other body fluids of healthy human subjects. Since the calprotectin concentration rises markedly in some inflammatory diseases including rheumatoid arthritis, this protein has been thought to be a marker of inflammatory disease. Recently, we identified calprotectin in human dental calculus and gingival crevicular fluid (GCF), and found that the calprotectin concentration in GCF from patients with periodontitis was significantly higher than that in GCF from healthy subjects. In the present study, the association of GCF calprotectin level with GCF volume, gingival index (GI), and levels of biochemical markers including collagenase and aspartate aminotransferase (AST) in GCF was investigated to clarify the relationship between GCF calprotectin level and periodontal inflammation.Methods: Ninety GCF samples collected from periodontal pockets with a probing depth of more than 4 mm in 54 patients with adult periodontitis were used for these assays. The GCF volume was measured, and GI in each site was recorded. The calprotectin content in GCF samples was determined by ELISA using a specific antibody. The activity of collagenase or AST was measured by a respective assay kit.Results: The total amount of calprotectin and GCF volume showed a highly significant correlation (r = 0.64, P