Expression and roles of Cl- channel ClC-5 in cell cycles of myeloid cells
Expression and roles of Cl- channel ClC-5 in cell cycles of myeloid cells
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DOI：
10.1016/j.bbrc.2004.03.036
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发表时间：
2004-04-23
影响因子：
3.1
通讯作者：
Inagaki, C
中科院分区：
文献类型：
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作者：
Jiang, BH;Hattori, N;Inagaki, C
CIC-5是已知在肾脏中表达的氯离子通道。我们以前报道过CIC-5 mRNA在未成熟的人髓系细胞（HL-60）中也有强表达，而在成熟的中性粒细胞中表达较弱。为了阐明其潜在机制，我们研究了CIC-5表达与细胞周期之间的关系。二甲基亚砜处理HL-60细胞可使其分化为G 0/G1期细胞，从而降低CIC-5 mRNA的表达。细胞分选和同步化实验表明，CIC-5 mRNA在S期和G2/M期表达量较高，而在G 0/G1期表达量较低。CIC-5反义寡核苷酸可抑制HL-60细胞增殖，降低CIC-5蛋白表达，可能与G2期阻滞有关。这些结果表明，CIC-5的细胞周期依赖性表达在骨髓细胞的细胞周期进程中具有作用。(C)2004年爱思唯尔公司All rights reserved.
CIC-5 is a chloride channel known to be expressed in the kidney. We previously reported that CIC-5 mRNA was also strongly expressed in immature human myeloid cell line (HL-60), but weakly expressed in mature neutrophils. To clarify the underlying mechanisms, we examined the relationship between CIC-5 expression and cell cycle. Dimethyl sulfoxide treatment of HL-60 that causes differentiation with G0/G1 cell cycle arrest decreased the expression of CIC-5 rnRNA. Cell sorting and synchronization experiments revealed that CIC-5 rnRNA expression was high in S and G2/M phases and low in G0/G1 phase. CIC-5 antisense oligonucleotide suppressed proliferation of HL-60 cells with a decrease in CIC-5 protein expression, probably due to G2 arrest. These results suggest that cell cycle-dependent expression of CIC-5 has a role in cell cycle progression in myeloid cells. (C) 2004 Elsevier Inc. All rights reserved.