Natural variation at OsCERK1 regulates arbuscular mycorrhizal symbiosis in rice
Natural variation at OsCERK1 regulates arbuscular mycorrhizal symbiosis in rice
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OsCERK1 的自然变异调节水稻丛枝菌根共生
DOI：
10.1111/nph.16158
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发表时间：
2019-10-03
期刊：
影响因子：
9.4
通讯作者：
Duanmu, Deqiang
中科院分区：
文献类型：
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作者：
Huang, Renliang;Li, Zheng;Duanmu, Deqiang
丛枝菌根真菌(AMF)与陆地植物之间的共生相互作用是有效获取和利用养分的关键。我们对控制AMF在水稻上定植的关键过程的了解仍然有限。东乡野生稻(DY)比水稻品种中早35(ZZ35)对不规则根结线虫的侵染能力更强。构建了染色体片段置换系，对DY的OsCERK1基因进行了定位。以中花11号(ZZ11)为材料，构建转基因植株，比较丛枝菌根真菌对水稻根部定殖的影响。与ZZ35相比，含有OsCERK1(DY)的染色体单片段代换系具有更高的含磷量和产量。在DY、ZZ35和ZH11的OsCERK1单倍型中发现了4个氨基酸替换，其中2个位于第二赖氨酸基序结构域，这是导致不同水稻品种AMF定殖水平差异的关键。异源OsCERK1(DY)在ZH11中的表达显著提高了AMF的定殖率，增加了对稻瘟病菌的抗性。值得注意的是，在4660个栽培稻品种中缺失了OsCERK1(DY)单倍型。我们得出结论，OsCERK1是一个影响与AMF共生互作的关键基因，OsCERK1(DY)具有提高水稻磷获取和利用效率的生物技术潜力，用于可持续农业。
The symbiotic interaction between arbuscular mycorrhizal fungi (AMF) and land plants is essential for efficient nutrient acquisition and utilisation. Our understanding of key processes controlling the AMF colonisation in rice is still limited. Dongxiang wild rice (DY) exhibited a stronger colonisation with Rhizophagus irregularis than the rice cultivar Zhongzao 35 (ZZ35). Chromosome segment substitution lines were constructed and the OsCERK1 gene from DY was mapped. Transgenic plants in the japonica rice Zhonghua 11 (ZZ11) were constructed to compare root colonisation by AMF. Chromosome single-segment substitution lines containing OsCERK1(DY) showed higher phosphorus content and grain yield relative to ZZ35. Four amino acids substitutions were identified among the OsCERK1 haplotypes of DY, ZZ35 and ZH11 and two of these were in the second lysine-motif domain, which is essential for the differences of AMF colonisation level among rice varieties. Heterologous expression of OsCERK1(DY) in ZH11 significantly enhanced AMF colonisation and increased resistance against the pathogenic fungi Magnaporthe oryzae. Notably, the OsCERK1(DY) haplotype was absent from 4660 cultivated rice varieties. We conclude that OsCERK1 is a key gene affecting the symbiotic interaction with AMF and OsCERK1(DY) has the biotechnological potential to increase rice phosphorus acquisition and utilisation efficiency for sustainable agriculture.