Lotus japonicus Clathrin Heavy Chain1 Is Associated with Rho-Like GTPase ROP6 and Involved in Nodule Formation
Lotus japonicus Clathrin Heavy Chain1 Is Associated with Rho-Like GTPase ROP6 and Involved in Nodule Formation
复制标题
莲子网格蛋白重链1与ROP6 GTP酶相关并参与结节形成
DOI：
10.1104/pp.114.256107
复制
发表时间：
2015-04-01
期刊：
影响因子：
7.4
通讯作者：
Zhang, Zhongming
中科院分区：
文献类型：
--
作者：
Wang, Chao;Zhu, Maosheng;Zhang, Zhongming
促红细胞生成因子受体（NFR）下游促红细胞生成因子信号传导的潜在机制尚未完全表征。在这项研究中，网格蛋白重链1（CHC 1）被证明与Rho样GTTR 0 P6相互作用，Rho样GTTR 0 P6是莲花中NFR 5的相互作用伴侣。CHC 1基因被发现在所有植物组织中组成型表达，并诱导中慢生根瘤菌感染的根毛和根瘤原基。当在本氏烟草（Nicotiana benthamiana）叶片中表达时，CHC 1和ROP 6共定位于细胞周边和细胞质点状结构内。In M.在感染根毛后，CHC蛋白在感染袋附近的细胞质点状结构中沿感染丝膜和寄主细胞质膜沿着分布。表达CHC 1-Hub结构域（网格蛋白介导的内吞作用的显性负效应子）的转基因植物被发现抑制了早期促红细胞生成基因的表达并损害了M。洛迪感染，导致排尿减少。酪氨酸磷酸酶抑制剂A23，网格蛋白介导的细胞质膜货物的内吞作用的抑制剂，治疗有类似的效果，下调早期的表达基因。这些发现表明网格蛋白在豆科植物与根瘤菌的共生中起着重要作用。
Mechanisms underlying nodulation factor signaling downstream of the nodulation factor receptors (NFRs) have not been fully characterized. In this study, clathrin heavy chain1 (CHC1) was shown to interact with the Rho-Like GTPase ROP6, an interaction partner of NFR5 in Lotus japonicus. The CHC1 gene was found to be expressed constitutively in all plant tissues and induced in Mesorhizobium loti-infected root hairs and nodule primordia. When expressed in leaves of Nicotiana benthamiana, CHC1 and ROP6 were colocalized at the cell circumference and within cytoplasmic punctate structures. In M. loti-infected root hairs, the CHC protein was detected in cytoplasmic punctate structures near the infection pocket along the infection thread membrane and the plasma membrane of the host cells. Transgenic plants expressing the CHC1-Hub domain, a dominant negative effector of clathrin-mediated endocytosis, were found to suppress early nodulation gene expression and impair M. loti infection, resulting in reduced nodulation. Treatment with tyrphostin A23, an inhibitor of clathrin-mediated endocytosis of plasma membrane cargoes, had a similar effect on down-regulation of early nodulation genes. These findings show an important role of clathrin in the leguminous symbiosis with rhizobia.