PULSATILE LH-RELEASE DURING THE OVULATORY LH SURGE ON PROESTRUS IN THE RAT
PULSATILE LH-RELEASE DURING THE OVULATORY LH SURGE ON PROESTRUS IN THE RAT
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DOI：
10.1095/biolreprod24.1.100
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发表时间：
1981-01-01
影响因子：
3.6
通讯作者：
GALLO, RV
中科院分区：
文献类型：
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作者：
GALLO, RV
在排卵LH [促黄体激素，促黄体生成素]峰期间，在发情前期1400 - 1930小时，通过颈静脉插管以10 μ l全血/2-3分钟的速率对具有4天发情周期的未麻醉大鼠放血。所有LH释放激增的大鼠均发生排卵。这些浪涌包括上升期、平台期和下降期。LH释放在平台期呈脉冲状，如与测定变异相比，个体出血期的变异系数百分比显著增加所示;在上升期和下降期，如血液LH水平明显的最低点至峰值变化（大于放射免疫测定法检测到的最小显著变化）所证明。血液LH水平在上升期增加，然后由于高振幅LH脉冲（上升期538 ng/ml，平台期572 ng/ml）而达到平台期，LH脉冲间期分别为16和23 min。血液LH水平下降，在下降阶段，因为脉冲幅度（386 ng/ml）显著降低，LH脉冲间期与上升相相比显著延长至29 min。大鼠动情前期排卵LH峰期发生脉冲式LH释放，LH峰期上升、平台期并且血液LH水平的下降可以通过在浪涌的不同阶段期间LH脉冲幅度和频率的变化来解释。
Unanesthetized rats with 4-day estrous cycles were bled through jugular vein cannulae at a rate of 10 .mu.l whole blood/2-3 min from 1400 to 1930 h on proestrus during the ovulatory LH [luteinizing hormone, lutropin] surge. Ovulation occurred in all rats that had surges of LH release. These surges consisted of ascending, plateau and descending periods. LH release was pulsatile during the plateau portion as indicated by a significant increase in the percent coefficient of variation for individual bleeding periods when compared with assay variation, and during the ascending and descending phases as evidenced by pronounced nadir-to-peak changes in blood LH levels which were greater than the minimum significant change detected by radioimmunoassay. Blood LH levels increased during the ascending phase and then plateaued because of high amplitude LH pulses (538 ng/ml in the ascending phase and 572 ng/ml in the plateau phase), which occurred with LH interpulse intervals of 16 and 23 min, respectively. Blood LH levels declined during the descending phase since the pulse amplitude (386 ng/ml) significantly decreased, and in comparison with the ascending phase the LH interpulse interval lengthened significantly to 29 min. Pulsatile LH release occurs during the ovulatory LH surge on proestrus in the rat, and that the rise, plateau, and fall in blood LH levels can be accounted for by changes in the LH pulse amplitude and frequency during different phases of the surge.