Centrally administered neuropeptide W-30 activates magnocellular neurosecretory cells in the supraoptic and paraventricular nuclei with neurosecretion in rats
Centrally administered neuropeptide W-30 activates magnocellular neurosecretory cells in the supraoptic and paraventricular nuclei with neurosecretion in rats
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DOI：
10.1677/joe.1.06636
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发表时间：
2006-08-01
影响因子：
4
通讯作者：
Ueta, Yoichi
中科院分区：
文献类型：
--
作者：
Kawasaki, Makoto;Onaka, Tatsushi;Ueta, Yoichi
我们用放射免疫分析法检测了静脉注射神经肽W-30（NPW 30）对血浆精氨酸加压素（AVP）和血浆催产素（OXT）的影响。用原位杂交组织化学方法观察c-fos mRNA、AVP hnRNA和Fos蛋白在视上核（SON）和室旁核（PVN）的表达。静脉注射NPW 30后5和15 min，血浆AVP和OXT均显著升高（2（.）8 nmol/大鼠）。原位杂交组织化学结果显示，在给药后15、30和60 h，SON和PVN中c-fos mRNA和AVP hnRNA的诱导显著增加。NPW30（1（.）4 nmol/大鼠）。Fos/AVP和Fos/OXT双重免疫染色显示，在静脉注射NPW 30后90 min，SON和PVN中的AVP样免疫反应（LI）细胞和OXT样免疫反应（LI）细胞均显示核Fos样LI（2（.）8 nmol/大鼠）。这些结果表明，中枢NPW 30可能参与了SON和PVN大细胞神经分泌细胞AVP和OXT分泌的调节。
We examined the effects of i.c.v. administration of neuropeptide W-30 (NPW30) on plasma arginine vasopressin (AVP) and plasma oxytocin (OXT) using RIA. The induction of c-fos mRNA, AVP heteronuclear (hn)RNA, and c-Fos protein (Fos) in the supraoptic nucleus (SON) and paraventricular nucleus (PVN) of rats were also investigated using hi situ hybridization histochemistry for c-fos mRNA and AVP hnRNA, and immunohistochemistry for Fos. Both plasma AVP and OXT were significantly increased at 5 and 15 min after i.c.v. administration of NPW30 (2(.)8 nmol/rat). In situ hybridization histochemistry revealed that the induction of c-fos mRNA and AVP hnRNA in the SON and PVN were significantly increased 15, 30, and 60 him after i.c.v. administration. of NPW30 (1(.)4 nmol/rat). Dual immunostaining for Fos/AVP and Fos/OXT revealed that both AVP-like innuunoreactive (LI) cells and OXT-LI cells exhibited nuclear Fos-LI in the SON and PVN, 90 min after i.c.v. administration of NPW30 (2(.)8 nmol/rat). These results suggest that central NPW30 may be involved in the regulation of secretion of AVP and OXT in the magnocellular neurosecretory cells in the SON and PVN.