Induction of adrenal tyrosine hydroxylase mRNA by single immobilization stress occurs even after splanchnic transection and in the presence of cholinergic antagonists.
Induction of adrenal tyrosine hydroxylase mRNA by single immobilization stress occurs even after splanchnic transection and in the presence of cholinergic antagonists.
复制标题
即使在内脏横断后且存在胆碱能拮抗剂的情况下，单一固定应激也会诱导肾上腺酪氨酸羟化酶 mRNA。
DOI：
10.1046/j.1471-4159.1996.66010138.x
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发表时间：
1996
影响因子：
4.7
通讯作者：
Sabban,EL
中科院分区：
文献类型：
--
作者：
Kvetnanský,R;Nankova,B;Hiremagalur,B;Viskupic,E;Vietor,I;Rusnak,M;McMahon,A;Kopin,IJ;Sabban,EL
制动应激可升高大鼠肾上腺血浆中的儿茶酚胺和酪氨酸羟化酶（TH）基因表达。本研究探讨了IMO诱导肾上腺TH mRNA水平变化的机制。肾上腺髓质的神经支配主要是胆碱能的，内脏神经切断术以及烟碱受体拮抗剂可防止冷诱导的TH mRNA水平升高。在这项研究中，IMO诱导的血浆儿茶酚胺升高，而不是TH mRNA水平，被拮抗剂氯异松达明降低。毒蕈碱拮抗剂阿托品也不能阻止IMO应激引起的TH mRNA升高。此外，通过单侧内脏神经切断术切除肾上腺神经并不能阻断IMO诱导的TH mRNA的升高，但完全阻止了神经肽Y mRNA的诱导。这些结果提示：（1）IMO应激引起的肾上腺TH基因表达的大量增加不受胆碱能受体或内脏神经支配的调节;（2）IMO应激时大鼠肾上腺髓质TH基因表达的增加与儿茶酚胺分泌的调节机制无关。
Immobilization (IMO) stress elevates plasma catecholamines and increases tyrosine hydroxylase (TH) gene expression in rat adrenals. This study examined the mechanism(s) of IMO‐induced changes in adrenal TH mRNA levels. Innervation of the adrenal medulla is predominantly cholinergic and splanchnicotomy as well as nicotinic receptor antagonists prevent the cold‐induced rise in TH mRNA levels. In this study, the IMO‐induced rise in plasma catecholamines, but not TH mRNA levels, was reduced by the antagonist chlorisondamine. Muscarinic antagonist atropine also did not prevent the IMO stress‐elicited rise in TH mRNA. Furthermore, denervation of the adrenals by unilateral splanchnicotomy did not block the IMO‐induced rise in TH mRNA but completely prevented the induction of neuropeptide Y mRNA. These results suggest that (1) the large increase in adrenal TH gene expression elicited by a single IMO stress is not regulated via cholinergic receptors or splanchnic innervation, and (2) there is a dissociation between regulatory mechanisms of catecholamine secretion and elevation of TH gene expression in the adrenal medulla of rats during IMO stress.