Intimal thickness associated with endothelial dysfunction in human vein grafts.
Intimal thickness associated with endothelial dysfunction in human vein grafts.
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DOI：
10.1016/j.jss.2012.06.017
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发表时间：
2013-03
影响因子：
2.2
通讯作者：
Komalavilas, Padmini
中科院分区：
文献类型：
--
作者：
Li, Fan Dong;Sexton, Kevin W.;Hocking, Kyle M.;Osgood, Michael J.;Eagle, Susan;Cheung-Flynn, Joyce;Brophy, Colleen M.;Komalavilas, Padmini
关键词：
内膜增生是一个复杂的过程，被认为是由损伤引起的，是静脉移植失败的主要原因。在这项研究中，我们假设人隐静脉的基底内膜厚度是内皮功能障碍和潜在的内膜增生的预测因子。在冠状动脉搭桥术中获得人隐静脉。用苯肾上腺素收缩节段，用卡巴考放松节段，以测定内皮依赖性松弛。静脉段用10%的福尔马林缓冲液固定，高血清培养14 d后再用福尔马林固定。用verhoefff - van Gieson染色固定组织，用光学显微镜和计算机图像分析系统计算平均内膜和内侧厚度。人隐静脉基底内膜厚度从18.80µm到241.3µm不等。血管内皮依赖性松弛度变化很大，范围从0到27.59%。基底内膜厚度大于120mm的人隐静脉内皮依赖性舒张（8.90±6.32%）明显低于基底内膜厚度小于120mm的人（21.97±10.64%）。内皮功能障碍与基底内膜厚度> 120µm相关（P = 0.02）。器官培养14天后，基底内膜厚度也与内膜厚度增加相关（P=0.0001）。基底内膜厚度大于120µm是内皮功能障碍的预测指标，并且由于基底内膜厚度越大也与器官培养后内膜厚度增加相关，因此基底内膜厚度可能预测由于内膜增生导致的静脉移植失败。
Intimal hyperplasia is a complex process thought to be initiated by injury and is the leading cause of vein graft failure. In this investigation we hypothesized that basal intimal thickness in human saphenous vein is a predictor of endothelial dysfunction and potentially intimal hyperplasia. Human saphenous veins were obtained during coronary artery bypass surgery. The segments were contracted with phenylephrine, and relaxed with carbachol to determine endothelial-dependent relaxation. Vein segments were fixed in 10% buffered formalin, and cultured for 14 days in high serum and then fixed in formalin. The fixed tissues were stained with Verhoeff-Van Gieson and average intimal and medial thicknesses were calculated using light microscopy and a computerized image analysis system. Human saphenous veins displayed variable amounts of basal intimal thickness ranging from 18.80 to 241.3 µm. Endothelial-dependent relaxation of the veins was highly variable with values ranging from 0 to 27.59%. Human saphenous vein with basal intimal thickness greater than 120mm had significantly less endothelial-dependent relaxation (8.90±6.32%) than those with basal intimal thickness less than 120mm (21.97±10.64%). Endothelial dysfunction correlated with basal intimal thickness > 120µm (P = 0.02). Basal intimal thickness also correlated with increased intimal thickness after 14 days in organ culture (P=0.0001). Basal intimal thickness greater than 120 µm is a predictor of endothelial dysfunction, and since greater basal intimal thickness also correlated with increased intimal thickness after organ culture, basal intimal thickness may predict vein graft failure due to intimal hyperplasia.
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影响因子：
4.3
作者：
KOMORI, K;GLOVICZKI, P;VANHOUTTE, PM
通讯作者：
VANHOUTTE, PM
影响因子：
4.6
作者：
Rousou, Laki J.;Taylor, Kristin B.;Thatte, Hemant S.
通讯作者：
Thatte, Hemant S.
影响因子：
9
作者：
LAWRIE, GM;MORRIS, GC;EARLE, N
通讯作者：
EARLE, N
影响因子：
4.3
作者：
Owens, Christopher D.
通讯作者：
Owens, Christopher D.
DOI：
10.1016/s1078-5884(96)80134-1
发表时间：
1996-01-01
影响因子：
5.7
作者：
Porter, KE;Varty, K;London, NJM
通讯作者：
London, NJM