Targeted transplantation of iron oxide-labeled, adipose-derived mesenchymal stem cells in promoting meniscus regeneration following a rabbit massive meniscal defect.
Targeted transplantation of iron oxide-labeled, adipose-derived mesenchymal stem cells in promoting meniscus regeneration following a rabbit massive meniscal defect.
复制标题
氧化铁标记的脂肪源性间充质干细胞的靶向移植促进兔巨大半月板缺损后的半月板再生
DOI：
10.3892/etm.2015.2944
复制
发表时间：
2016-02
影响因子：
2.7
通讯作者：
Feng G
中科院分区：
文献类型：
--
作者：
Qi Y;Yang Z;Ding Q;Zhao T;Huang Z;Feng G
修复巨大的半月板缺损在临床上仍然是一个挑战。然而，靶向磁性细胞输送是一种新兴的技术，可能对其治疗有用。本研究旨在确定超顺磁性氧化铁(SPIO)标记的脂肪间充质干细胞(ASCs)关节内靶向注射对兔大面积半月板损伤模型的影响。可以直接标记ASCs，24小时后几乎100%的ASCs被SPIO标记，这些标记的ASCs可以通过磁铁定位。核磁共振成像(MRI)可以检测到SPIO标记的ASCs的离心沉淀物。切除18只新西兰大白兔内侧半月板的前半部分。7天后，将兔随机分为3组，分别注射2×106个SPIO标记的ASCs、2×106个未标记ASCs或生理盐水。将永磁体固定于手术关节外1d，术后6周、12周取材，行MRI、大体及组织学观察和普鲁士蓝染色。MRI检测半月板SPIO阳性细胞引起的明显低信号伪影。组织学观察显示，SPIO-ASCs组半月板的前部与自然组织相似，表现为典型的纤维软骨细胞，周围有较丰富的细胞外基质。连接界面和新生半月板的富胶原基质与宿主半月板结合良好。此外，所有组均有退行性改变，但关节内注射SPIO-ASCs或ASCs可减轻这些退行性改变。普鲁士蓝染色显示，移植的ASCs与再生组织直接相关。总体而言，有针对性的关节内注射SPIO-ASCs促进了半月板的再生，同时提供了对骨关节损伤的保护作用。
Repair of a massive meniscal defect remains a challenge in the clinic. However, targeted magnetic cell delivery, an emerging technique, may be useful in its treatment. The present study aimed to determine the effect of targeted intra-articular injection of superparamagnetic iron oxide (SPIO)-labeled adipose-derived mesenchymal stem cells (ASCs) in a rabbit model of a massive meniscal defect. ASCs may be directly labeled and almost 100% of the ASCs were labeled with SPIO after 24 h; these SPIO-labeled ASCs may be orientated by magnet. The centrifuged SPIO-labeled ASCs precipitations may be detected by magnetic resonance imaging (MRI). The anterior half of the medial meniscus of 18 New Zealand Rabbits was excised. After 7 days, the rabbits were randomized to injections of 2×106 SPIO-labeled ASCs, 2×106 unlabeled ASCs or saline. Permanent magnets were fixed to the outside of the operated joints for one day, and after 6 and 12 weeks, the knee joints were examined using MRI, gross and histological observation, and Prussian blue staining. Marked hypointense artifacts caused by SPIO-positive cells in the meniscus were detected using MRI. Histological observation revealed that the anterior portion of the meniscus was similar to the native tissue, demonstrating typical fibrochondrocytes surrounded by richer extracellular matrix in the SPIO-ASCs group. Collagen-rich matrix bridging the interface and the neo-meniscus integrated well with its host meniscus. Furthermore, degenerative changes occurred in all groups, but intra-articular injection of SPIO-ASCs or ASCs alleviated these degenerative changes. Prussian blue staining indicated that the implanted ASCs were directly associated with the regenerated tissue. Overall, targeted intra-articular delivery of SPIO-ASCs promoted meniscal regeneration whilst providing protective effects from osteoarthritic damage.