The cDNA structure, expression, and chromosomal assignment of the mouse Fas antigen.
The cDNA structure, expression, and chromosomal assignment of the mouse Fas antigen.
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DOI：
10.4049/jimmunol.148.4.1274
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发表时间：
1992-02
影响因子：
4.4
通讯作者：
Rie Watanabe‐Fukunaga;C. Brannan;N. Itoh;S. Yonehara;N. Copeland;N. Jenkins;S. Nagata
中科院分区：
文献类型：
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作者：
Rie Watanabe‐Fukunaga;C. Brannan;N. Itoh;S. Yonehara;N. Copeland;N. Jenkins;S. Nagata
细胞表面Fas抗原是一种介导细胞凋亡的膜相关多肽。从小鼠巨噬细胞系BAM3 mRNA构建的cDNA文库中分离到Fas抗原的cDNA克隆。小鼠Fas抗原的核苷酸序列和推导出的氨基酸序列与人Fas抗原cDNA的同源性分别为58.5%和49.3%。小鼠Fas抗原由306个氨基酸组成，计算Mr为34,971，并包含一个跨膜结构域，将分子分为细胞外结构域和细胞质结构域。在小鼠胸腺、心脏、肝脏和卵巢中检测到编码Fas抗原的2.1 kb mRNA，但在脑和脾脏中未检测到。ifn - γ可显著诱导小鼠成纤维细胞L929和巨噬细胞BAM3细胞系中Fas抗原基因的表达，而ifn - α / β对Fas抗原基因表达无显著影响。种间回交分析表明，Fas抗原编码基因位于小鼠第19染色体远端。
The cell surface Fas antigen is a membrane-associated polypeptide which can mediate apoptosis. cDNA clones encoding the Fas antigen were isolated from a cDNA library constructed with mRNA from the mouse macrophage cell line BAM3. The nucleotide sequence and the deduced amino acid sequence of the mouse Fas antigen were 58.5 and 49.3% identical, respectively, to the corresponding sequences of human Fas antigen cDNA. The mouse Fas antigen consists of 306 amino acids with a calculated Mr of 34,971 and contains a single transmembrane domain which divides the molecule into extracellular and cytoplasmic domains. A 2.1-kb mRNA coding for the Fas antigen was detected in the mouse thymus, heart, liver, and ovary but not in brain and spleen. The expression of the Fas antigen gene in mouse fibroblast L929 and macrophage BAM3 cell lines was significantly induced by treatment with IFN-gamma but not by IFN-alpha/beta. Interspecific backcross analysis indicated that the gene coding for the Fas antigen is in the distal region of mouse chromosome 19.