Involvement of brain opioid receptors in the anti-allodynic effect of hyperbaric oxygen in rats with sciatic nerve crush-induced neuropathic pain.
Involvement of brain opioid receptors in the anti-allodynic effect of hyperbaric oxygen in rats with sciatic nerve crush-induced neuropathic pain.
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DOI：
10.1016/j.brainres.2013.08.050
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发表时间：
2013-11-06
期刊：
影响因子：
2.9
通讯作者：
Quock RM
中科院分区：
文献类型：
--
作者：
Gibbons CR;Liu S;Zhang Y;Sayre CL;Levitch BR;Moehlmann SB;Shirachi DY;Quock RM
早期的研究表明，高压氧(HBO2)可以在急性疼痛模型中产生抗伤害感受性作用。最近的研究表明，高压氧也可以在慢性疼痛的动物模型中产生疼痛缓解作用。本研究的目的是确定高压氧治疗是否可以抑制神经病理性疼痛大鼠的痛觉过敏，以及阿片类拮抗剂纳曲酮(NTX)是否可以阻断这一作用。雄性SD大鼠在麻醉状态下挤压坐骨神经，用冯-弗雷电子麻醉仪测定机械阈值。绘制不同治疗组高压氧抗痛觉异常作用的时程曲线，并测定各治疗组的曲线下面积(AUC)。神经挤压后7天，大鼠在3.5个绝对大气压(ATA)下用HBO2处理60min，与单纯神经挤压对照大鼠相比，显示出抗痛觉过敏的作用。在高压氧治疗前24小时，另一组大鼠被植入阿尔采特®渗透性微泵，该微泵将NTX持续释放到侧脑室7天。这些注射了NTX的高压氧治疗的大鼠表现出与只接受神经挤压的大鼠相当的超常反应。AUC数据分析显示，HBO2可显著减轻神经挤压所致的痛觉超敏；NTX可逆转HBO2的这一抗痛觉异常的作用。这些结果表明阿片受体参与了高压氧的止痛作用。
Earlier research has demonstrated that hyperbaric oxygen (HBO2) can produce an antinociceptive effect in models of acute pain. Recent studies have revealed that HBO2 can produce pain relief in animal models of chronic pain as well. The purpose of the present investigation was to ascertain whether HBO2 treatment might suppress allodynia in rats with neuropathic pain and whether this effect might be blocked by the opioid antagonist naltrexone (NTX). Male Sprague Dawley rats were subjected to a sciatic nerve crush under anesthesia and mechanical thresholds were assessed using an electronic von Frey anesthesiometer. The time course of the HBO2-induced anti-allodynic effect in different treatment groups was plotted, and the area-under-the-curve (AUC) was determined for each group. Seven days after the nerve crush procedure, rats were treated with HBO2 at 3.5 atmospheres absolute (ATA) for 60 min and exhibited an anti-allodynic effect, compared to nerve crush-only control rats. Twenty-four hours before HBO2 treatment, another group of rats was implanted with Alzet® osmotic minipumps that continuously released NTX into the lateral cerebral ventricle for 7 days. These NTX-infused, HBO2-treated rats exhibited an allodynic response comparable to that exhibited by rats receiving nerve crush only. Analysis of the AUC data showed that HBO2 significantly reduced the nerve crush-induced allodynia; this anti-allodynic effect of HBO2 was reversed by NTX. These results implicate opioid receptors in the pain relief induced by HBO2.
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影响因子：
4.1
作者：
PILCHER, WH;JOSEPH, SA;MCDONALD, JV
通讯作者：
MCDONALD, JV
影响因子：
2.9
作者：
Quock LP;Zhang Y;Chung E;Ohgami Y;Shirachi DY;Quock RM
通讯作者：
Quock RM
影响因子：
6.6
作者：
Dall'Era, Marc A.;Hampson, Neil B.;Corman, John M.
通讯作者：
Corman, John M.
影响因子：
1.7
作者：
Ohgami, Yusuke;Zylstra, Carlyn C.;Quock, Raymond M.
通讯作者：
Quock, Raymond M.
影响因子：
2.9
作者：
Wilson, Hilary D.;Wilson, Judy R.;Fuchs, Perry N.
通讯作者：
Fuchs, Perry N.