Consumption of an acid protease derived from Aspergillus oryzae causes bifidogenic effect in rats
Consumption of an acid protease derived from Aspergillus oryzae causes bifidogenic effect in rats
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DOI：
10.1016/j.nutres.2017.06.004
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发表时间：
2017-08-01
影响因子：
4.5
通讯作者：
Kato, Norihisa
中科院分区：
文献类型：
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作者：
Yang, Yongshou;Iwamoto, Akane;Kato, Norihisa
在喂食补充有Amano蛋白酶制剂（来源于Aspergillusplasma）的高脂肪饮食的大鼠盲肠中发现双歧杆菌丰度显著升高。蛋白酶制剂含有几种消化酶，包括酸性蛋白酶（AcP）、碱性蛋白酶和淀粉酶。我们推测，通过天野蛋白酶制剂提高双歧杆菌的丰度与蛋白酶制剂中涉及的消化酶有关。为了验证这一假设，本研究进行了调查，如果这种生物遗传效应是因为酸性磷酸酶。给大鼠喂食含有从Amano蛋白酶制剂获得的纯化AcP的高脂肪饮食2周。日粮中添加1g/kg Amano蛋白酶可显著提高大鼠盲肠和粪便中双歧杆菌的数量。双歧杆菌数量不受补充纯化的AcP（0.096 g/kg）的影响，其水平相当于1 g/kg Amano蛋白酶饮食中发现的AcP量。当大鼠喂食含有0.384 g/kg AcP的饮食（AcP的量是1 g/kg Amano蛋白酶饮食中所用的量的4倍）时，盲肠和粪便中的双歧杆菌数量以及盲肠中的乳酸水平显著升高（P <0.05）。因此，1 g/kg Amano蛋白酶饲料的生物遗传效应不能用AcP解释。然而，有趣的是，发现补充的AcP在比1 g/kg Amano蛋白酶饮食中使用的剂量高4倍的剂量下引起显著的生物合成效应。(C)2017爱思唯尔公司All rights reserved.
A marked elevation in the abundance of Bifidobacterium was found in the cecum of rats that were fed a high-fat diet supplemented with an Amano protease preparation (derived from Aspergillus oryzae). The protease preparation contains several digestive enzymes, including acid protease (AcP), alkaline protease, and amylase. We hypothesized that the elevation in the abundance of Bifidobacterium by Amano protease preparation is associated with the digestive enzymes involved in the protease preparation. To test this hypothesis, this study was conducted to investigate if such bifidogenic effect is because of the AcP. Rats were fed a high-fat diet containing purified AcP obtained from the Amano protease preparation for 2 weeks. The numbers of Bifidobacterium in the cecum and feces of rats were markedly elevated by the dietary supplementation of 1 g/kg Amano protease. Bifidobacterium numbers were unaffected by supplementation with purified AcP (0.096 g/kg) at the level equivalent to the AcP amount found in the 1-g/kg Amano protease diet. Bifidobacterium numbers in the cecum and feces, and lactate levels in the cecum were significantly (P < .05) elevated when rats were fed a diet containing 0.384 g/kg AcP (4-fold higher amount of AcP than that used in the 1-g/kg Amano protease diet). Thus, the bifidogenic effect of 1 g/kg Amano protease diet could not be explained by the AcP. However, intriguingly, supplemental AcP was found to cause a significant bifidogenic effect at the dose that is 4-fold higher than that used in the 1-g/kg Amano protease diet. (C) 2017 Elsevier Inc. All rights reserved.