Embryonic handedness choice in C. elegans involves the Gα protein GPA-16
Embryonic handedness choice in C. elegans involves the Gα protein GPA-16
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DOI：
10.1242/dev.00839
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发表时间：
2003-12-01
期刊：
影响因子：
4.6
通讯作者：
Wood, WB
中科院分区：
文献类型：
--
作者：
Bergmann, DC;Lee, M;Wood, WB
左-右（LR）轴的极性最初以正确的手性建立的机制对于任何胚胎都不清楚。线虫胚胎表现出LR不对称性与不变的手性，这是第一次明显的六细胞阶段，并持续整个发展。我们在这里表明，一个强大的功能丧失突变基因最初指定的spn-1影响早期纺锤体的方向，并导致近随机化的手性选择。这种突变与编码局部皮质成分的三个par基因的突变在遗传上相互作用。我们发现，spn-1基因编码的Ga蛋白GPA-16，这似乎是所需的中心体协会的G β蛋白。我们以后将这个基因称为gpa-16。这些结果首次证明了异源三聚体G蛋白参与胚胎LR不对称性的建立，并提示了它们可能的作用方式。
The mechanism by which polarity of the left-right (LR) axis is initially established with the correct handedness is not understood for any embryo. C elegans embryos exhibit LR asymmetry with an invariant handedness that is first apparent at the six-cell stage and persists throughout development. We show here that a strong loss-of-function mutation in a gene originally designated spn-1 affects early spindle orientations and results in near randomization of handedness choice. This mutation interacts genetically with mutations in three par genes that encode localized cortical components. We show that the spn-1 gene encodes the Galpha protein GPA-16, which appears to be required for centrosomal association of a Gbeta protein. We will henceforth refer to this gene as gpa-16. These results demonstrate for the first time involvement of heterotrimeric G proteins in establishment of embryonic LR asymmetry and suggest how they might act.