3-DIMENSIONAL STRUCTURE OF RECOMBINANT HUMAN INTERFERON-GAMMA
3-DIMENSIONAL STRUCTURE OF RECOMBINANT HUMAN INTERFERON-GAMMA
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DOI：
10.1126/science.1902591
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发表时间：
1991-05-03
期刊：
影响因子：
56.9
通讯作者：
BUGG, CE
中科院分区：
文献类型：
--
作者：
EALICK, SE;COOK, WJ;BUGG, CE
重组人干扰素-γ的X-射线晶体结构已被确定与使用多同晶置换技术。干扰素-γ在溶液中是二聚体，在不对称单元中与两个通过非结晶学二重轴相关的二聚体一起结晶。该蛋白主要是α-螺旋，每个亚基中有6个螺旋，占结构的62%;没有β-折叠。人干扰素-γ的二聚体结构通过具有多个亚基间相互作用的亚基界面上的螺旋缠绕而稳定。
The x-ray crystal structure of recombinant human interferon-gamma has been determined with the use of multiple-isomorphous-replacement techniques. Interferon-gamma, which is dimeric in solution, crystallizes with two dimers related by a noncrystallographic twofold axis in the asymmetric unit. The protein is primarily alpha-helical, with six helices in each subunit that comprise approximately 62 percent of the structure; there is no beta-sheet. The dimeric structure of human interferon-gamma is stabilized by the intertwining of helices across the subunit interface with multiple intersubunit interactions.