An assessment of adult mosquito collection techniques for studying species abundance and diversity in Maferinyah, Guinea
An assessment of adult mosquito collection techniques for studying species abundance and diversity in Maferinyah, Guinea
复制标题
DOI：
10.1186/s13071-020-04023-3
复制
发表时间：
2020-03-24
影响因子：
3.2
通讯作者：
Walker, Thomas
中科院分区：
文献类型：
--
作者：
Cansado-Utrilla, Cintia;Jeffries, Claire L.;Walker, Thomas
媒介控制规划通常采用几种蚊虫收集方法。然而，它们针对不同的行为，导致对物种多样性和丰度的估计存在偏差。鉴于西非缺乏诱蚊器数据，我们比较了几内亚五种诱蚊器组合和人类着陆捕蚊器（hlc）的效果。方法2018年6 - 7月在几内亚3个村庄采用5 × 5拉丁广场设计，对CDC光诱法（LT）、BG哨诱法（BG2T）、重力诱法（GT）和隐形诱法（ST）进行比较。ST是一种便携式陷阱，其性能与LT相似，但结合了led和白炽灯，由于尚未进行广泛测试，因此被包括在内。用BG2T和BG、MB5引诱剂代替CO2引诱剂进行引诱效果试验。hlc进行了5晚，但不作为拉丁广场的一部分。采用广义线性混合模型比较诱捕器、地点和采集时间对蚊虫丰度的影响。物种鉴定采用pcr分析和Sanger测序。结果5个诱蚊器共捕获蚊虫10610只。ST诱蚊器捕获的蚊虫数量（7096只）明显高于其他诱蚊器，但造成的蚊虫损坏数量也高于其他诱蚊器。ST区和BG2T区分别采集到最多的冈比亚按蚊和埃及伊蚊。hlc主要捕获An。黄花苜蓿（41%）和黄花苜蓿杂交种。冈比亚和安哥拉。与此形成对比的是5个圈闭，主要捕获安。米拉(83%)。与Fandie（半农村）和Maferinyah Centre I（半城市）相比，农村站点（Senguelen）的蚊子丰度和总体多样性最高。我们的研究结果首次在几内亚证实了四种寄生虫的存在。结论ST诱蚊器捕获的蚊虫数量最多，提示该诱蚊器在几内亚和西非类似地区的蚊虫监测中可能发挥重要作用。我们建议在昆虫学研究中纳入分子工具，因为它们已经帮助鉴定了该地区的25种蚊子。
Background Several mosquito collection methods are routinely used in vector control programmes. However, they target different behaviours causing bias in estimation of species diversity and abundance. Given the paucity of mosquito trap data in West Africa, we compared the performance of five trap-lure combinations and Human Landing Catches (HLCs) in Guinea. Methods CDC light traps (LT), BG sentinel 2 traps (BG2T), gravid traps (GT) and Stealth traps (ST) were compared in a 5 x 5 Latin Square design in three villages in Guinea between June and July 2018. The ST, a portable trap which performs similarly to a LT but incorporates LEDs and incandescent light, was included since it has not been widely tested. BG2T were used with BG and MB5 lures instead of CO2 to test the efficacy of these attractants. HLCs were performed for 5 nights, but not as part of the Latin Square. A Generalised Linear Mixed Model was applied to compare the effect of the traps, sites and collection times on mosquito abundance. Species identification was confirmed using PCR-based analysis and Sanger sequencing. Results A total of 10,610 mosquitoes were captured across five traps. ST collected significantly more mosquitoes (7096) than the rest of the traps, but resulted in a higher number of damaged specimens. ST and BG2T collected the highest numbers of Anopheles gambiae (s.l.) and Aedes aegypti mosquitoes, respectively. HLCs captured predominantly An. coluzzii (41%) and hybrids of An. gambiae and An. coluzzii (36%) in contrast to the five traps, which captured predominantly An. melas (83%). The rural site (Senguelen) presented the highest abundance of mosquitoes and overall diversity in comparison with Fandie (semi-rural) and Maferinyah Centre I (semi-urban). Our results confirm the presence of four species for the first time in Guinea. Conclusions ST collected the highest number of mosquitoes suggesting this trap may play an important role for mosquito surveillance in Guinea and similar sites in West Africa. We recommend the incorporation of molecular tools in entomological studies since they have helped to identify 25 mosquito species in this area.