Towards mimicking natural protein channels with aligned carbon nanotube membranes for active drug delivery.
Towards mimicking natural protein channels with aligned carbon nanotube membranes for active drug delivery.
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DOI：
10.1016/j.lfs.2009.04.006
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发表时间：
2010-04-10
期刊：
影响因子：
6.1
通讯作者：
Hinds, Bruce J.
中科院分区：
文献类型：
--
作者：
Majumder, Mainak;Stinchcomb, Audra;Hinds, Bruce J.
碳纳米管（CNT）膜提供了一个令人兴奋的机会来模拟天然蛋白质通道，这是由于1）显著增强流体流动的机制2）在孔入口处放置“看门人”化学的能力3）在看门人分子上发生生化反应的能力和4）独立地化学官能化膜的每一侧的能力。制造对齐的CNT膜，并且用看门人化学修饰CNT孔入口。压力驱动的流体流动和扩散实验进行了研究通过碳纳米管的传输机制。通过CNT膜的传输机制主要是1）接近本体预期的离子扩散2）主要由于镜面反射而使气体流动增强1-2个数量级3）由于接近理想的滑移边界界面而使流体流动比常规材料快4-5个数量级。运输可以通过在孔入口处使用空间位阻、静电吸引/排斥或生物化学状态的“看门人”化学来调节。带电束缚分子的构象可以通过施加偏压来调节，从而为可编程药物释放装置设置阶段。膜结构在机械上比脂质双层膜更坚固，允许基于蛋白质通道的原理进行大规模化学分离，递送或传感。蛋白质通道的性能比常规膜材料快几个数量级。模拟蛋白质通道的基本要求存在于CNT膜系统中。
Carbon nanotube (CNT) membranes offer an exciting opportunity to mimic natural protein channels due to 1) a mechanism of dramatically enhanced fluid flow 2) ability to place ‘gatekeeper’ chemistry at the entrance to pores 3) the ability for biochemical reactions to occur on gatekeeper molecules and 4) an ability to chemically functionalize each side of the membrane independently. Aligned CNT membranes were fabricated and CNT pore entrances modified with gatekeeper chemistry. Pressure driven fluid flow and diffusion experiments were performed to study the mechanisms of transport through CNTs. The transport mechanism through CNT membranes is primarily 1) ionic diffusion near bulk expectation 2) gas flow enhanced 1–2 orders of magnitude primarily due to specular reflection 3) fluid flow 4–5 orders of magnitude faster than conventional materials due to a nearly ideal slip-boundary interface. The transport can be modulated by ‘gatekeeper’ chemistry at the pore entrance using steric hindrance, electrostatic attraction/repulsion, or biochemical state. The conformation of charged tethered molecules can be modulated by applied bias setting the stage for programmable drug release devices. The membrane structure is mechanically far more robust than lipid bilayer films, allowing for large-scale chemical separations, delivery or sensing based on the principles of protein channels. The performance of protein channels is several orders of magnitude faster than conventional membrane materials. The fundamental requirements of mimicking protein channels are present in the CNT membrane system.
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影响因子：
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