Spinal cord afferent systems containing the nerve terminal protein NT75.
Spinal cord afferent systems containing the nerve terminal protein NT75.
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脊髓传入系统含有神经末梢蛋白 NT75。
DOI：
10.1002/cne.903320205
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Coulter,JD
中科院分区：
文献类型：
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作者：
Kanekar,SS;Ritchie,TC;Coulter,JD
在成年脊髓，NT75的免疫细胞化学染色集中在背角浅层的神经末梢。背角深层有中等程度的免疫细胞化学标记，而腹角仅有少量染色。应用毁损技术、秋水仙碱处理、逆行追踪结合免疫细胞化学染色等方法，对含NT75的脊神经末梢的来源进行了研究。初级传入神经元表达NT75免疫反应，主要分布在背角浅层的浓染和深板层的部分标记。结果发现，皮质脊髓和几乎所有向脊髓下行投射的脑干神经元也表达NT75免疫反应，包括那些终止于腹角的神经元。秋水仙碱治疗脊髓也导致NT75染色在大多数，如果不是全部，脊髓神经元。脊髓传入的三个主要来源(初级感觉系统、下行系统和内在系统)的神经元似乎都能表达NT75免疫反应，但一些神经元的神经末梢中通常含有较高水平的NT75蛋白。先前对发育脊髓的分析显示，在出生后早期，广泛密集的NT75标记遍及脊髓灰质，后来仅限于成人模式。这些研究支持这样的假设，即许多脊髓通路在发育过程中表达高水平的NT75免疫反应性，但只有某些通路在成人中保持高水平。©1993 Wiley-Liss，Inc.
In the adult spinal cord, immunocytochemical staining for NT75 is concentrated in nerve terminals in the superficial laminae of the dorsal horn. Deeper laminae of the dorsal horn contain moderate immunocytochemical labeling, but the ventral horn is only sparsely stained. The origin of spinal nerve terminals containing NT75 was investigated with lesion techniques, colchicine treatment, and retrograde tracing in combination with immunocytochemical staining. Primary afferent neurons express NT75 immunoreactivity and account for most of the dense staining in the superficial dorsal horn and part of the labeling in the deeper laminae. It was found that corticospinal and virtually all brainstem neurons with descending projections to the spinal cord also express NT75 immunoreactivity, including those terminating in the ventral horn. Colchicine treatment of the spinal cord also resulted in NT75 staining in most, if not all, spinal neurons. It appears that neurons in all three major sources of spinal afferents (primary sensory, descending, and intrinsic systems) can express NT75 immunoreactivity, but that some neurons normally contain higher levels of the protein in their nerve terminals. Previous analysis of developing spinal cord has shown widespread, dense NT75 labeling throughout the spinal gray in the early postnatal period, which later becomes restricted to the adult pattern. These studies support the hypothesis that many spinal pathways express high levels of NT75 immunoreactivity during development, but that only certain pathways maintain high levels in the adult. © 1993 Wiley‐Liss, Inc.