Salivary peptidome profiling for diagnosis of severe early childhood caries.
Salivary peptidome profiling for diagnosis of severe early childhood caries.
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DOI：
10.1186/s12967-016-0996-4
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发表时间：
2016-08-15
影响因子：
7.4
通讯作者：
Zheng S
中科院分区：
文献类型：
--
作者：
Sun X;Huang X;Tan X;Si Y;Wang X;Chen F;Zheng S
儿童早期重度龋病S在儿童中有相当高的患病率，是一个广泛存在的问题，在发展中国家和发达国家都有重大影响。目前，临床筛查S癌时，尚无早期检测技术和诊断试验具有较高的敏感性和特异性。本研究应用基于磁珠(MB)的基质辅助激光解吸电离飞行时间质谱仪(MALDI-TOFMS)筛选S胆囊癌的候选标志物，以建立其蛋白质谱和诊断模型。首先，我们利用上述技术分别在治疗前、治疗后1周和4周的时间点检测了10例S-ECC患儿唾液中的特异性表达多肽。在此基础上，采用K近邻方法建立了S-食管癌的诊断模型，并在下一阶段的研究中对另外6名儿童进行了验证。然后用线性离子陷阱-轨道捕获-质谱仪(LTQ-Orbitrap-MS)鉴定唾液中哪些蛋白质可能是这些多肽的来源。比较3个时间点，发现有7个多肽峰有显著差异，其中S-ECC组治疗前2个较治疗后升高，其余5个较治疗后降低。我们建立的诊断模型的灵敏度和特异度均为83.3%。其中两个多肽被鉴定为唾液中一种重要的分泌性蛋白-组蛋白-1的片段。因此，我们证实了基于MB的MALDI-TOF MS是从青少年S口腔癌患者唾液中筛选特异性多肽的有效方法，组蛋白-1可能是这种常见牙病的一个重要的候选生物标志物。这些发现对建立S胆囊癌新的诊断方法具有很好的应用前景。本文的在线版本(doi：10.1186/s12967-0160996-4)包含补充材料，授权用户可以使用。
Severe early childhood caries (s-ECC), which has quite high prevalence among children, is a widespread problem with significant impacts among both developing and developed countries. At present, it is widely known that no early detective techniques and diagnostic tests could have high sensitivity and specificity when using for clinical screening of s-ECC. In this study, we had applied magnetic bead (MB)-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to screen distinctive candidate biomarkers of this disease, so as to establish protein profiles and diagnostic models of s-ECC. Firstly, we used the technique mentioned above to detect specifically expressed peptides in saliva samples from ten children with s-ECC, separately at the time point of before, 1 and 4 weeks after dental treatment. Then a diagnostic model for s-ECC was established with the K nearest-neighbour method, which was validated in another six children in the next stage of study. After that, linear ion trap-orbitrap-mass spectrometry (LTQ-Orbitrap-MS) was performed to identify which of the proteins in saliva might be the origination of these peptides. We found that seven peptide peaks were significantly different when comparing the three time points, among them two were higher, while other five were lower in the pre-treatment s-ECC group compared with post-treatment. The sensitivity and specificity of the diagnostic model we built were both 83.3 %. Two of these peptides were identified to be segments of histatin-1, which was one important secretory protein in saliva. Hereby we confirmed that MB-based MALDI-TOF MS is an effective method for screening distinctive peptides from the saliva of junior patients with s-ECC, and histatin-1 may probably be one important candidate biomarker of this common dental disease. These findings might have bright prospect in future in establishing new diagnostic methods for s-ECC. The online version of this article (doi:10.1186/s12967-016-0996-4) contains supplementary material, which is available to authorized users.