Zinc uptake by isolated rat hepatocytes.
Zinc uptake by isolated rat hepatocytes.
复制标题
离体大鼠肝细胞对锌的吸收。
DOI：
10.1016/0005-2736(81)90099-7
复制
发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Klaassen,CD
中科院分区：
文献类型：
--
作者：
Stacey,NH;Klaassen,CD
分离的大鼠肝细胞被用来研究在早期暴露时间的锌的摄取。将肝细胞与65 Zn（1-500 μM）孵育，并在25 s至60 min的时间范围内取出样品。观察到摄取的双相模式，第一相摄取快速，随后是较慢的第二相。第一阶段的吸收速率与底物浓度呈非线性关系，而第二阶段的吸收速率与底物浓度呈线性关系。10 μM镉的存在仅使第一相的吸收速度降低。这表明第一阶段至少部分是载体介导的，而没有迹象表明载体参与第二阶段。KCN（1 mM）和羰基氰间氯苯腙（2 μM）未引起65 Zn（1 μM）摄取的任何变化，这表明锌摄取中没有活性成分。
Isolated rat hepatocytes were used to investigate the uptake of zinc at early exposure times. Hepatocytes were incubated with 65 Zn (1–500 μM) and samples were withdrawn at times ranging from 25 s to 60 min. A biphasic pattern of uptake was observed with a rapid first phase of uptake followed by a slower second phase. The relationship between velocity of uptake and substrate concentration for the first phase was nonlinear, while that of the second phase was linear. The presence of 10 μM cadmium produced a decrease in the velocity of uptake of only the first phase. This suggests that the first phase is at least partly carrier mediated, while there is no indication of involvement of a carrier in the second phase. KCN (1 mM) and carbonyl cyanide m-chlorophenylhydrazone (2 μM), did not cause any change in the uptake of 65 Zn (1 μM), which suggests that there is no active component in the uptake of zinc.