SELECTION FOR ACID TOLERANCE IN RHIZOBIUM-MELILOTI
SELECTION FOR ACID TOLERANCE IN RHIZOBIUM-MELILOTI
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DOI：
10.1007/bf02376781
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发表时间：
1988-01-01
期刊：
影响因子：
4.9
通讯作者：
DANTUONO, MF
中科院分区：
文献类型：
--
作者：
HOWIESON, JG;EWING, MA;DANTUONO, MF
In the selection of acid-tolerant Rhizobium meliloti, procedures for the collection and isolation of rhizobia, and the assessment of acid tolerance, have not been critically evaluated. Such procedures form the basis of this study. Root nodules were collected from Medicago spp. found growing on acid soil in Sardinia [Italy]. Their encumbent bacteria were isolated directly onto media adjusted over a range of pH values, and then assessed for acid tolerance in both the laboratory and field. Strains of Rhizobium meliloti isolated onto low pH media were, in general, more acid-tolerant than sister isolates from high pH media, when tested in both the laboratory and field. Dilution (10 or 100 fold) of the inocula used in the laboratory assessment did not greatly influence the rating derived, although there was some effect of bacterial colony type on growth rating. The link between polysaccharide production and acid tolerance was not strong. There was a poor correlation between the growth ratings derived from the laboratory screening and acid tolerance as expressed in the field.
