Transcription of thymic stromal lymphopoietin via Toll-like receptor 2 in canine keratinocytes: a possible association of Staphylococcus spp. in the deterioration of allergic inflammation in canine atopic dermatitis.
Transcription of thymic stromal lymphopoietin via Toll-like receptor 2 in canine keratinocytes: a possible association of Staphylococcus spp. in the deterioration of allergic inflammation in canine atopic dermatitis.
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DOI：
10.1111/vde.12301
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发表时间：
2016-03
影响因子：
1.4
通讯作者：
M. Sakamoto;R. Asahina;H. Kamishina;S. Maeda
中科院分区：
文献类型：
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作者：
M. Sakamoto;R. Asahina;H. Kamishina;S. Maeda
背景葡萄球菌的定植、过度生长和随后的感染。在犬特应性皮炎（CAD）中经常观察到，其导致皮肤炎症的强度。葡萄球菌参与CAD发病的机制尚不清楚。研究表明，胸腺基质淋巴细胞生成素（TSLP），一种由葡萄球菌属的细胞壁成分诱导的细胞因子，可能在包括CAD发病机制在内的Th 2应答中起关键作用。假设/目的为了确定合成的三酰化脂肽（TLR 1/2配体），葡萄球菌属的细胞壁成分，在犬角质形成细胞中通过TLR 2诱导TSLP的转录。方法用合成的三酰化脂肽刺激犬角质形成细胞系后，再用靶向小干扰RNA抑制TLR 2后，定量TSLP的转录。结果合成的三酰化脂肽刺激6 h后TSLP的转录增强，TLR 2的敲低可完全抑制TSLP的转录。结论和临床意义结果表明，葡萄球菌属的合成细胞壁成分。通过TLR 2在犬角质形成细胞中诱导TSLP的转录。将需要进行额外的研究，以调查葡萄球菌属是否。通过TLR 2介导的TSLP产生促进CAD中的Th 2应答。
BACKGROUND Colonization, overgrowth and subsequent infection by Staphylococcus spp. is frequently observed in canine atopic dermatitis (CAD), where it contributes to the intensity of cutaneous inflammation. The mechanisms by which staphylococci contribute to the pathogenesis of CAD are unclear. Studies suggest that thymic stromal lymphopoietin (TSLP), a cytokine induced by a cell wall component of Staphylococcus spp., may play a critical role in Th2 responses including the pathogenesis of CAD. HYPOTHESIS/OBJECTIVE To determine if synthetic triacylated lipopeptide (TLR1/2 ligand), a cell wall component of Staphylococcus spp., induces the transcription of TSLP via TLR2 in canine keratinocytes. METHODS Transcription of TSLP was quantified in a canine keratinocyte cell line after stimulation with synthetic triacylated lipopeptide, and again after inhibition of TLR2 by a targeted small interfering RNA. RESULTS The transcription of TSLP was enhanced 6 h after stimulation with the synthetic triacylated lipopeptide; it was completely suppressed by knockdown of TLR2. CONCLUSIONS AND CLINICAL IMPORTANCE The results demonstrated that a synthetic cell wall component of Staphylococcus spp. induced transcription of TSLP via TLR2 in canine keratinocytes. Additional studies will be required to investigate whether Staphylococcus spp. contributes to Th2 responses in CAD through TLR2-mediated TSLP production.