Multiplex RT-PCR for Rapid Detection of Viruses Commonly Causing Diarrhea in Pediatric Patients
Multiplex RT-PCR for Rapid Detection of Viruses Commonly Causing Diarrhea in Pediatric Patients
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DOI：
10.1002/jmv.24711
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发表时间：
2017-05-01
影响因子：
12.7
通讯作者：
Ushijima, Hiroshi
中科院分区：
文献类型：
--
作者：
Thongprachum, Aksara;Khamrin, Pattara;Ushijima, Hiroshi
采用5组引物建立了多重RT-PCR方法，用于检测轮状病毒A、B和C、腺病毒、星状病毒、诺如病毒GI和GII、沙波病毒、爱知病毒、双埃可病毒、肠道病毒、柯萨奇病毒、博卡病毒和藏红花病毒。用751份日本急性腹泻患儿粪便标本对该方法的灵敏度进行了评价和测试。751份粪便标本中528份（70.3%）检出多种病毒。对临床标本中不同病毒的混合感染也能有效检测。该方法灵敏度高、特异性强、结果可靠，可用于常规诊断。(C)2016 Wiley Periodicals，Inc.
Multiplex RT-PCR method using five sets of panel primers was developed for the detection of diarrheal viruses, including rotavirus A, B, and C, adenovirus, astrovirus, norovirus GI and GII, sapovirus, Aichi virus, parechovirus, enterovirus, cosavirus, bocavirus, and Saffold virus. The sensitivity of the method was evaluated and tested with 751 fecal specimens collected from Japanese children with acute diarrhea. Several kinds of viruses were detected in 528 out of 751 (70.3%) fecal specimens. Mixed-infection with different viruses in clinical specimens could also be effectively detected. The method proved to be reliable with highly sensitive and specific and useful for routine diagnosis. (C) 2016 Wiley Periodicals, Inc.