Dietary aromatase inhibitor treatment converts XY gsdf homozygous mutants to sub-fertile male in Nile tilapia (Oreochromis niloticus)
Dietary aromatase inhibitor treatment converts XY gsdf homozygous mutants to sub-fertile male in Nile tilapia (Oreochromis niloticus)
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DOI：
10.1016/j.aquaculture.2023.739381
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发表时间：
2023-02
期刊：
影响因子：
4.5
通讯作者：
Sen Fan;Hong‐Juan Shi;You-Xing Peng;Yuanqing Huang;Mouyan Jiang;Guang-Li Li-Guang-Li-Li-2151297544;De-shou Wang;
中科院分区：
文献类型：
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作者：
Sen Fan;Hong‐Juan Shi;You-Xing Peng;Yuanqing Huang;Mouyan Jiang;Guang-Li Li-Guang-Li-Li-2151297544;De-shou Wang;
Gonadal soma-derived factor (gsdf) belongs to the TGF-β superfamily and is critical for sex determination and differentiation in fish. XYgsdf−/−Nile tilapia (Oreochromis niloticus) show male-to-female sex reversal 1 month after hatching (mah) and are infertile at the adult age; however, the functions ofgsdfin spermatogenesis in fish are unclear. Herein, XYgsdf−/−Nile tilapia were converted to males with testis by feeding 200 μg/g letrozole (LE), an aromatase inhibitor, from 1 to 4 mah. The testis somatic index of XYgsdf−/−LE fish was significantly lower than that of XYgsdf+/+LE fish at 6 mah. The artificial fertilization ratio of XYgsdf−/−LE fish sperm (53.22 ± 4.35%) was significantly lower than that of XYgsdf+/+LE fish (86.33 ± 1.53%), indicating that XYgsdf−/−LE male fish were sub-fertile. Testes of LE XYgsdf−/−andgsdf+/+fish at 6 mah were used for a transcriptome analysis. Compared with the testis of XYgsdf+/+LE fish, 1426 and 1654 genes were up-regulated and down-regulated in the testis of XYgsdf−/−LE individuals, respectively. A KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway enrichment analysis revealed that the differentially expressed genes (DEGs) were engaged in 21 pathways, including cancer, cell growth and death, and endocrine system pathways. Genes related to steroidogenesis and meiosis, such ascyp19a1a,foxl2,andcyp17a1,were significantly up-regulated, whereassycp3,dmc1,andaldh1a1were significantly down-regulated in the testis. In addition, a serum steroid hormone analysis showed that 17β-estradiol and 11-ketotestosterone levels of XYgsdf−/−LE fish were significantly higher and lower than those of XYgsdf+/+LE fish. These findings demonstrated that endocrine system disruption in XYgsdf−/−LE might explain the decrease in fertility. This research establishes the groundwork for future research on the function ofgsdfin spermatogenesis in fish.
