Folic Acid Functionalized PFBT Fluorescent Polymer Dots for Tumor Imaging
Folic Acid Functionalized PFBT Fluorescent Polymer Dots for Tumor Imaging
复制标题
用于肿瘤成像的叶酸功能化 PFBT 荧光聚合物点
DOI：
10.1002/cjoc.201500780
复制
发表时间：
2016-06-01
影响因子：
5.4
通讯作者：
Xiong, Liqin
中科院分区：
文献类型：
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作者：
Cao, Fengwen;Xiong, Liqin
采用纳米沉淀法制备了叶酸功能化的PFBT-COOH和PFBT-NH 2聚合物点，并首次展示了其在体外和体内肿瘤成像中的应用。合成的FA-PFBT-COOH聚合物点的直径为50-60 nm，在水中的ζ电位大于-30 mV，而合成的FA-PFBT-NH 2聚合物点的直径为90-105 nm，在水中的ζ电位大于-20 mV。此外，FA-PFBT-COOH聚合物点在水溶液中以及在细胞中产生比FA-PFBT-NH 2聚合物点更强的荧光强度，并且能够在静脉内注射后对活小鼠中的H1299肿瘤成像。该研究显示了FA-PFBT-COOH聚合物点作为生物医学成像荧光纳米探针的巨大潜力。
Folic acid functionalized PFBT-COOH and PFBT-NH2 polymer dots were prepared using a nano precipitation method, and their applications for tumor imaging in vitro and in vivo were demonstrated for the first time. The synthesized FA-PFBT-COOH polymer dots were 50–60 nm in diameter with a zeta potential of greater than −30 mV in water, while the synthesized FA-PFBT-NH2 polymer dots were 90–105 nm in diameter with a zeta potential of greater than −20 mV in water. Furthermore, the FA-PFBT-COOH polymer dots produced stronger fluorescence intensity in water solution as well as in the cells than the FA-PFBT-NH2 polymer dots, and were able to image H1299 tumors in living mice after intravenous injection. This study showed the great potentials of FA-PFBT-COOH polymer dots as fluorescent nanoprobes for biomedical imaging.