FISH analysis of 142 EGFP transgene integration sites into the mouse genome
FISH analysis of 142 EGFP transgene integration sites into the mouse genome
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DOI：
10.1006/geno.2002.7008
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发表时间：
2002-12-01
期刊：
影响因子：
4.4
通讯作者：
Okabe, M
中科院分区：
文献类型：
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作者：
Nakanishi, T;Kuroiwa, A;Okabe, M
转基因动物的生产是研究各种生物过程的重要技术。然而，特定转基因的整合是否在小鼠基因组中随机发生尚未确定。通过荧光原位杂交分析我们生产的142个EGFP（绿色荧光蛋白的突变体）转基因系的整合位点，表明转基因已整合到每个小鼠染色体中。在82.4%的品系中观察到单一整合位点。15例（10.6%）同时整合到两个不同的基因位点。在3例转基因创始小鼠中，整合位点出现嵌合体（2.1%）。染色体易位7例（4.9%）。此外，当我们对这些小鼠品系的转基因整合位点进行统计分析时，发现它们在整个基因组中分布不均匀。这是第一个通过生产100多个转基因小鼠品系来分析转基因整合位点的报告。
Production of transgenic animals is an important technique for studying various biological processes. However, whether the integration of a particular transgene occurs randomly in the mouse genome has not been determined. Analysis by fluorescence in situ hybridization of the integration sites of the 142 EGFP (a mutant of green fluorescent protein) transgenic lines that we produced showed that the transgenes had become incorporated into every mouse chromosome. A single integration site was observed in 82.4% of the lines. The concomitant integrations of transgene into two different loci were observed in 15 cases (10.6%). In 3 cases, the transgenic founder mice showed chimerism in integration sites (2.1%). Chromosomal translocation was observed in 7 cases (4.9%). Moreover, when we statistically analyzed the transgene integration sites of these mouse lines, they were shown to distribute unevenly throughout the genome. This is the first report to analyze the transgene integration sites by producing more than 100 transgenic mouse lines.