Intercalation-Driven Formation of siRNA Nanogels for Cancer Therapy
Intercalation-Driven Formation of siRNA Nanogels for Cancer Therapy
复制标题
用于癌症治疗的siRNA纳米凝胶的嵌入驱动形成
DOI：
10.1021/acs.nanolett.1c03539
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发表时间：
2021-11-24
期刊：
影响因子：
10.8
通讯作者：
Tao, Wei
中科院分区：
文献类型：
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作者：
Huang, Xiangang;Wu, Gongwei;Tao, Wei
RNA干扰（RNAi）是治疗包括癌症在内的各种疾病的有效方法。基于小干扰RNA（siRNA）的治疗的临床转化需要安全且有效的递送载体。在这里，我们报告了一种基于siRNA纳米凝胶（NG）的递送载体，其直接由核酸双嵌入剂和siRNA分子之间的嵌入驱动。基于嵌入的siRNA NG具有良好的生理稳定性，并且可以通过不同的内吞途径有效地进入细胞。此外，siRNA NG不仅可以在体外沉默靶基因，而且可以在体内显著抑制肿瘤生长。因此，本研究为开发用于癌症治疗的siRNA递送平台提供了基于嵌入的策略。据我们所知，这是第一个嵌入驱动的siRNA NG的报告。
RNA interference (RNAi) is a powerful approach in the treatment of various diseases including cancers. The clinical translation of small interfering RNA (siRNA)-based therapy requires safe and efficient delivery vehicles. Here, we report a siRNA nanogels (NG)-based delivery vehicle, which is driven directly by the intercalation between nucleic acid bis-intercalator and siRNA molecules. The intercalation-based siRNA NG exhibits good physiological stability and can enter cells efficiently via different endocytosis pathways. Furthermore, the siRNA NG can not only silence the target genes in vitro but also significantly inhibit the tumor growth in vivo. Therefore, this study provides an intercalation-based strategy for the development of a siRNA delivery platform for cancer therapy. To the best of our knowledge, this is the first report of the intercalation-driven siRNA NG.