Effect of epinephrine on rat diaphragm.
Effect of epinephrine on rat diaphragm.
复制标题
肾上腺素对大鼠膈肌的影响。
DOI：
10.1016/s0021-9258(18)56223-2
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发表时间：
1950
期刊：
影响因子：
--
通讯作者：
E. Walaas
中科院分区：
文献类型：
--
作者：
O. Walaas;E. Walaas
肾上腺素对肌肉的特定作用包括糖原的加速分解，导致6-磷酸己糖的积累和无机磷酸盐的相应减少。这在离体青蛙肌肉的实验中最为明显，其中乳酸形成速率的继发性增加不是一个突出特征（1）。在完整的哺乳动物中，己糖B-磷酸在肌肉中的积累伴随着乳酸从肌肉中流出。肾上腺素的另一个作用是降低肌肉中葡萄糖的摄取率（2）。最近Somogyi（3）在人体实验中再次证实了这一点，并提出了一个问题，即这是肾上腺素的直接作用，还是通过增加垂体前叶分泌间接介导的。注射肾上腺素可促进垂体前叶分泌促肾上腺激素，这一点已被清楚地证明[4]。Cohen（5）得出结论，这种影响是间接的，因为注射肾上腺素会导致正常大鼠膈肌无氧糖酵解减少，而切除垂体的大鼠则不会。本文报道了在有氧条件下大鼠膈肌碳水化合物平衡的实验;它们表明，在体外加入肾上腺素对葡萄糖摄取、糖原形成和乳酸产生有显著影响。作为这项工作的必要准备，对微量糖原的测定进行了重新研究。
The specific effect of epinephrine on muscle consists of an accelerated breakdown of glycogen which results in an accumulation of hexose-6-phosphate and a corresponding decrease in inorganic phosphate. This is most clearly seen in experiments with isolated frog muscle in which a secondary increase in the rate of lactic acid formation is not a prominent feature (1). In intact mammals the accumulation of hexoseB-phosphate in muscle is accompanied by an outflow of lactic acid from muscle. Another effect of epinephrine which has received attention is a decreased rate of uptake of glucose in muscle (2). This has recently been reaffirmed by Somogyi (3) in experiments on man. The question has been raised whether this is a direct effect of epinephrine or whether it is mediated indirectly through increased anterior pituitary secretion. That injection of epinephrine elicits the secretion of adrenotropic hormone from the anterior pituitary has been clearly demonstrated (4). Cohen (5) concluded that the effect was an indirect one, since injection of epinephrine caused decreased anaerobic glycolysis in diaphragms from normal but not from hypophysectomized rats.In the present paper are reported experiments on carbohydrate balance on rat diaphragm under aerobic conditions; they show that addition of epinephrine in vitro has a marked effect on glucose uptake, glycogen formation, and lactic acid production. As a necessary preliminary to this work the determination of micro quantities of glycogen has been reinvestigated.