Aerobic degradation of 2-picolinic acid by a nitrobenzene-assimilating strain: Streptomyces sp. Z2.
Aerobic degradation of 2-picolinic acid by a nitrobenzene-assimilating strain: Streptomyces sp. Z2.
复制标题
DOI：
10.1016/j.biortech.2008.10.017
复制
发表时间：
2009-03
影响因子：
11.4
通讯作者：
Chunli Zheng;Ji-ti Zhou;Jing Wang;Baocheng Qu;Hong Lu;Hongxia Zhao
中科院分区：
文献类型：
--
作者：
Chunli Zheng;Ji-ti Zhou;Jing Wang;Baocheng Qu;Hong Lu;Hongxia Zhao
从硝基苯污染的活性污泥中分离到一株链霉菌Z2，该菌在好氧条件下以硝基苯为唯一碳源、氮源和能量源。结果表明，菌株Z2除对硝基苯外，还能降解2-吡啶甲酸。菌株Z2分别在36、50、72、100、136和180 h内完全降解初始浓度为500、1000、1500、2000、2500和3000 mg/L的2-吡啶甲酸。用Andrews方程描述了2-吡啶甲酸的降解动力学。动力学参数如下：qmax=3.81h-1，Ks=83.10mg/L，Ki=252.11mg/L。在降解过程中，Z2将2-吡啶甲酸转化为6-羟基吡啶甲酸，经紫外-可见光谱、核磁共振氢谱和质谱鉴定其为6-羟基吡啶甲酸。6-然后羟基吡啶甲酸裂解并矿化，释放氨。
Streptomyces sp. Z2 was isolated from nitrobenzene contaminated activated sludge, which utilized nitrobenzene as a sole source of carbon, nitrogen, and energy under aerobic condition. It was found that besides nitrobenzene strain Z2 can degrade 2-picolinic acid. Strain Z2 completely degraded 2-picolinic acid with initial concentration of 500mg/L, 1000mg/L, 1500mg/L, 2000mg/L, 2500mg/L, and 3000mg/L within 36h, 50h, 72h, 100h, 136h, and 180h, respectively. Kinetics of 2-picolinic acid degradation was described using the Andrews equation. The kinetic parameters were as follows: qmax=3.81h−1, Ks=83.10mg/L, and Ki=252.11mg/L. During the biodegradation process, Z2 transformed 2-picolinic acid into a product which was identified as 6-hydroxy picolinic acid by UV–vis spectrometry,1H nuclear magnetic resonance spectroscopy, and mass spectrometry. 6-Hydroxy picolinic acid was then cleaved and mineralized with release of ammonia.