Subclass-specific formation of perineuronal nets around parvalbumin-expressing GABAergic neurons in the Ammon’s horn of the mouse hippocampus.
Subclass-specific formation of perineuronal nets around parvalbumin-expressing GABAergic neurons in the Ammon’s horn of the mouse hippocampus.
复制标题
小鼠海马阿蒙角中表达小清蛋白的 GABA 能神经元周围神经元周围网的亚类特异性形成。
作者：
Yamada J;Jinno S
神经元周网（PNN）与小清蛋白阳性（PV+）神经元密切相关，并在控制发育神经可塑性方面发挥重要作用。考虑到PV+神经元分类的最新进展，在这里，我们的目的是澄清PNNs是否可能与海马中PV+神经元的特定亚类相关。在这项研究中，我们用紫藤花凝集素（WFA）标记PNN，并根据细胞位置，分子表达（神经肽Y [NPY]，生长抑素[SOM]，特殊的富含AT的序列结合蛋白-1 [SATB 1]）和通过立体定位注射Fluoro-Gold（FG）到内侧隔的逆行追踪的组合对PV+神经元进行分类。每个亚类的标准可以总结如下：轴突-轴突细胞，在皮层中的PV+/SATB 1-/NPY-细胞;篮状细胞，在皮层中的PV+/SATB 1 +/NPY-细胞;双层细胞，在皮层中的PV+/SATB 1 +/NPY+细胞;定向-腔隙-分子（O-LM）细胞，在定向层中的PV+/SOM+/FG-细胞;在定向层中，有许多细胞为前房-隔投射（H-S）细胞和PV+/SOM+/FG+细胞。根据光学分割原理估计PV+神经元周围WFA+ PNN形成的比例。绝大多数（超过90%）推定的PV+篮状细胞被PNN包围，而只有少数（小于10%）推定的PV+轴-轴、O-LM和H-S细胞被PNN包围。在假定的PV+双层细胞周围形成PNN的比率是中等的（25-50%）。这些发现表明PNNs可能与PV+篮细胞特异性相关，也为理解PNNs和PV+神经元在海马中的功能意义提供了一把钥匙。J. Comp.神经元523：790-804，2015.© 2015威利期刊公司.
Perineuronal nets (PNNs) are closely associated with parvalbumin‐positive (PV+) neurons, and play a major role in controlling developmental neural plasticity. Considering the recent advances in classification of PV+ neurons, here we aimed to clarify whether PNNs might be associated with specific subclasses of PV+ neurons in the hippocampus. In this study, we labeled PNNs byWisteria floribundaagglutinin (WFA), and classified PV+ neurons based on the combination of cellular location, molecular expression (neuropeptide Y [NPY], somatostatin [SOM], special AT‐rich sequence‐binding protein‐1 [SATB1]), and retrograde tracing through stereotaxic injection of Fluoro‐Gold (FG) into the medial septum. The criteria of each subclass can be summarized as follows: axo‐axonic cells, PV+/SATB1–/NPY– cells in the stratum pyramidale; basket cells, PV+/SATB1+/NPY– cells in the stratum pyramidale; bistratified cells, PV+/SATB1+/NPY+ cells in the stratum pyramidale; oriens‐lacunosum‐moleculare (O‐LM) cells, PV+/SOM+/FG– cells in the stratum oriens; hippocampo‐septal projection (H‐S) cells, and PV+/SOM+/FG+ cells in the stratum oriens. The ratios of formation of WFA+ PNNs around each subclass of PV+ neurons were estimated according to the optical disector principle. The vast majority (over 90%) of putative PV+ basket cells were surrounded by PNNs, while only a minor population (less than 10%) of putative PV+ axo‐axonic, O‐LM, and H‐S cells were enwrapped with PNNs. The ratios of formation of PNNs around putative PV+ bistratified cells were intermediate (25–50%). These findings indicate that PNNs may be specifically associated with PV+ basket cells, and also provide a key to understand the functional significance of PNNs and PV+ neurons in the hippocampus. J. Comp. Neurol. 523:790–804, 2015. © 2015 Wiley Periodicals, Inc.