Myocyte-specific enhancer factor 2A is essential for zebrafish posterior somite development.
Myocyte-specific enhancer factor 2A is essential for zebrafish posterior somite development.
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肌细胞特异性增强因子 2A 对于斑马鱼后体节发育至关重要。
DOI：
10.1016/j.mod.2006.07.005
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发表时间：
2006
影响因子：
2.6
通讯作者：
Song,Houyan
中科院分区：
文献类型：
--
作者：
Wang,Yuexiang;Qian,Linxi;Dong,Yongxin;Jiang,Qiu;Gui,Yonghao;Zhong,TaoP;Song,Houyan
有些人的发育与遗传因素密切相关。在斑马鱼的大规模突变筛选中，没有突变与肌细胞增强因子2A （MEF2A）位点相关。在本研究中，我们发现MEF2A敲除胚胎尾部弯曲向下，后体呈u型。此外，我们证明了MEF2A是Hedgehog信号传导所必需的。MEF2A抑制诱导后体细胞凋亡。我们进一步发现，Hedgehog信号可以负向调节体细胞中MEF2A的表达。微阵列研究揭示了许多在MEF2A突变体中差异表达的基因。我们的研究表明，MEF2A对斑马鱼后体细胞发育至关重要。
Somite development is governed tightly by genetic factors. In the large-scale mutagenesis screens of zebrafish, no mutations were linked to myocyte enhancer factor 2A (MEF2A) locus. In this study, we find that MEF2A knock-down embryos display a downward tail curvature and have U-shaped posterior somites. Furthermore, we demonstrate that MEF2A is required for Hedgehog signaling. MEF2A inhibition results in induction of apoptosis in the posterior somites. We further find that Hedgehog signaling can negatively regulate MEF2A expression in the somites. Microarray studies reveal a number of genes that are differentially expressed in the MEF2A morphants. Our studies suggest that MEF2A is essential for zebrafish posterior somite development.