Clonal analysis of the response of human myeloid leukemic cell lines to colony-stimulating activity.
Clonal analysis of the response of human myeloid leukemic cell lines to colony-stimulating activity.
复制标题
人骨髓白血病细胞系对集落刺激活性的反应的克隆分析。
DOI：
10.1182/blood.v58.2.285.bloodjournal582285
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发表时间：
1981
期刊：
影响因子：
20.3
通讯作者：
R. Gallo
中科院分区：
文献类型：
--
作者：
F. Ruscetti;S. Collins;A. Woods;R. Gallo
最近发展的两种对CsA有反应的人髓系白血病细胞系(HL-60和KG-1)为详细研究CsA与白血病髓系细胞的相互作用提供了机会。在这里，我们报告了HL-60和KG-1在延长细胞培养寿命期间的集落形成能力。几种不同来源的不同纯度的人CsA促进了这些细胞的集落形成。从SV-40转化的人滋养层细胞的条件培养液中获得的CsA被部分纯化，其对正常骨髓的活性与刺激HL-60集落形成的活性相同。开发了100多个HL-60克隆，并测试了它们对CsA的反应。所有细胞对CsA的反应都表现为菌落大小和数量的增加。然而，尽管许多化学物质可以诱导HL-60细胞分化，但100个克隆中的任何一个克隆都没有形成对CsA诱导分化的克隆。由于HL-60在没有添加任何外源性刺激因子的情况下形成自发集落，因此对HL-60条件培养液和细胞提取物进行了测试，以确定这些细胞产生自身的内源性促生长活性(如CsA样分子)。未发现促生长内源性活性，刺激正常骨髓或HL-60集落形成，即使采用浓缩和分级方法。这些实验表明：(1)CsA的作用明显有利于这些细胞系的增殖而不是分化；(2)CsA不太可能抑制HL-60和KG-1所代表的那种类型的白血病髓系细胞的生长；(3)如果HL-60细胞产生自己的促生长因子，则在培养液中检测不到它。
The recent development of two continuously proliferating human myeloid leukemic cell lines (HL-60 and KG-1) that response to CSA provides an opportunity for a detailed study of the interaction of CSA with leukemic myeloid cells. Here we report on the colony-forming ability of HL-60 and KG-1 over an extended culture life of the cells. Several different sources of human CSA of different stages of purity enhanced colony formation of these cells. CSA, obtained from conditioned media from an SV-40 transformed human trophoblast, was partially purified, and its activity for normal bone marrow copurified with the activity that stimulated HL-60 colony formation. Over 100 clones of HL-60 were developed and tested for their response to CSA. All responded to CSA by showing an increase in colony size and number. However, none of the colonies formed from any of the 100 clones differentiated in response to CSA despite the fact that many chemical can induce differentiation of HL-60. since HL-60 forms spontaneous colonies without the addition of any exogenous stimulating factors, HL-60 conditioned media and cell extracts were tested for the production by these cells of their own endogenous growth-promoting activity (such as a CSA-like molecule). No growth-promoting endogenous activity was found that stimulated normal bone marrow or HL-60 colony formation even after concentration and fractionation methods were employed. These experiments suggest that: (1) the effect of CSA markedly favors proliferation over differentiation in these cell lines; (2) CSA is unlikely to suppress growth of the age of the type of leukemic myeloid cells that HL-60 and KG-1 represent; and (3) if HL-60 cells produce their own growth-promoting factor it is not detectable in the media.