Shake It Off! Acoustic Manipulation of Lipid Vesicles for Mass Spectrometric Analysis
Shake It Off! Acoustic Manipulation of Lipid Vesicles for Mass Spectrometric Analysis
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DOI：
10.1021/acs.analchem.3c01722
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发表时间：
2023-08-30
影响因子：
7.4
通讯作者：
Evans-Nguyen，Theresa
中科院分区：
文献类型：
--
作者：
Taylor，Ashton N.;Huang，Yuqi;Evans-Nguyen，Theresa
分析脂类集合，包括脂质体和细胞外小泡(EVS)，由于它们的大小、不同的组成和聚集的趋势，是具有挑战性的。这种囊泡以简单的磷脂双层膜形成，在药物发现和传递中发挥着重要作用。质谱仪(MS)的使用允许对不同类别的脂类进行广泛的分析；然而，它们从更高级别的结构聚集体中释放出来通常是通过化学手段实现的。高频声表面波(SAW)机械破碎法是一种备受欢迎的脂泡制备方法，可用于MS采样。在这项工作中，用于破坏脂质体的SAW允许通过使用带有电晕放电(CD)电离的SAW雾化来直接分析其组成的脂类。我们探讨了持续时间、频率和非极性脂类(包括胆固醇)的掺入对SAW破坏脂质体的能力的影响。我们还报告了对脂质体衍生的脂类和溶液中的细胞色素C进行成功的MS分析，从而展示了在水样和自然MS条件下的应用。
Analyzing lipid assemblies, including liposomes and extracellular vesicles (EVs), is challenging due to their size, diverse composition, and tendency to aggregate. Such vesicles form with a simple phospholipid bilayer membrane, and they play important roles in drug discovery and delivery. The use of mass spectrometry (MS) allows for broad analysis of lipids from different classes; however, their release from the higher order structural aggregates is typically achieved by chemical means. Mechanical disruption by high frequency surface acoustic waves (SAW) is presented as an appealing alternative to preparing lipid vesicles for MS sampling. In this work, SAWs used to disrupt liposomes allow for the direct analysis of their constituent lipids by employing SAW nebulization with corona discharge (CD) ionization. We explore the effects of duration, frequency, and incorporation of nonpolar lipids, including cholesterol, on the SAW’s ability to disrupt the liposome. We also report on the successful MS analysis of liposome-derived lipids along with cytochrome C in solution, thus demonstrating applications to aqueous samples and native MS conditions.