Maintenance of granulocyte numbers during acute peritonitis is defective in galectin-3-null mutant mice
Maintenance of granulocyte numbers during acute peritonitis is defective in galectin-3-null mutant mice
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DOI：
10.1046/j.1365-2567.1998.00517.x
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发表时间：
1998-07-01
期刊：
影响因子：
6.4
通讯作者：
Poirier, F
中科院分区：
文献类型：
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作者：
Colnot, C;Ripoche, MA;Poirier, F
半乳糖凝集素-3，也被称为巨噬细胞标志物Mac-2，是一个结构相关的动物凝集素家族的成员，对β -半乳糖苷具有特异性。为了研究半乳糖凝集素-3在急性炎症中的作用，我们比较了野生型和半乳糖凝集素-3缺失突变小鼠腹腔注射巯基乙酸肉汤后的白细胞数量。注射后第1天，我们发现腹腔内单核吞噬细胞和粒细胞的募集没有差异。然而，注射巯基乙酸后4天，半乳糖凝集素-3突变小鼠与野生型小鼠相比，可恢复的粒细胞数量明显减少。由于突变的粒细胞没有表现出加速的凋亡速度，巨噬细胞对它们的摄取似乎不受突变的影响，本文描述的表型表明，在急性炎症的消退过程中，半乳糖凝集素-3参与了额外的控制。
Galectin-3, also known as the macrophage marker Mac-2, is a member of a family of structurally related animal lectins that exhibit specificity for beta-galactosides. In order to investigate the role of galectin-3 in acute inflammation, we have compared the number of leucocytes present in the peritoneal cavity of wild type and galectin-3 null mutant mice after intraperitoneal (i.p.) injection of thioglycolate broth. At day 1 after injection, we found no difference in the recruitment of mononuclear phagocytes and granulocytes to the peritoneal cavity. However, 4 days after thioglycolate injection, galectin-3 mutant mice exhibited a significantly reduced number of recoverable granulocytes compared to wild-type animals. As mutant granulocytes did not exhibit an accelerated rate of apoptosis and their uptake by macrophages appeared to be unaffected by the mutation, the phenotype described here suggests that galectin-3 participates in an additional level of control during the resolution of acute inflammation.