Commitment of 3T3-F442A cells to adipocyte differentiation takes place during the first 24-36 h after adipogenic stimulation:: TNF-α inhibits commitment
Commitment of 3T3-F442A cells to adipocyte differentiation takes place during the first 24-36 h after adipogenic stimulation:: TNF-α inhibits commitment
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DOI：
10.1016/s0014-4827(02)00036-8
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发表时间：
2003-04-01
影响因子：
3.7
通讯作者：
Kuri-Harcuch, W
中科院分区：
文献类型：
--
作者：
Castro-Muñozledo, F;Beltrán-Langarica, A;Kuri-Harcuch, W
我们研究了3T3-F442A细胞在促脂血清或生长激素刺激下的作用。融合的3T3-F442A前脂肪细胞用成脂培养基孵育，增加孵育次数；评估脂肪簇的数量、GPDH活性和脂质积累。结果表明，在脂肪生成条件下，细胞承诺发生在刺激后的第一个24-36小时。然后，通过选择性增殖承诺细胞，培养物的总细胞数量增加2倍，随后集落形成能力急剧下降，GPDH活性提高300至1000倍。细胞承诺不受胰岛素调节，但这种激素刺激了承诺细胞的克隆扩增和脂肪生成。低至5纳克/毫升浓度的tnf - α和视黄酸均可抑制生殖功能。结果表明，tnf - α在两种不同水平上抑制脂肪转化：当浓度低至5 ng/ml时，它会阻断承诺；当浓度达到100 ng/ml或更高时，细胞因子似乎会阻断细胞承诺后的有丝分裂扩张和其他分化步骤。确定细胞发育的特定时间将有助于研究可能调节细胞重编程为脂肪细胞的早期基因。(C) 2003 Elsevier Science（美国）版权所有。
We studied the commitment of 3T3-F442A cells during stimulation with adipogenic serum or growth hormone. Confluent 3T3-F442A preadipocytes were incubated with adipogenic medium for increasing times; the number of adipose clusters, GPDH activity, and lipid accumulation were evaluated. Results show that cell commitment took place during the first 24-36 It after stimulation under adipogenic conditions. Then, cultures underwent a 2-fold increase in total cell number through selective multiplication of committed cells, followed by a dramatic decrease in colony-forming ability and 300- to 1000-fold raise in GPDH activity. Cell commitment was not modulated by insulin, but this hormone stimulated clonal expansion of committed cells and lipogenesis. Commitment was inhibited by TNF-alpha at concentrations as low as 5 ng/ml, and by retinoic acid. The results show that TNF-alpha inhibits adipose conversion at two different levels: at concentrations as low as 5 ng/ml, it blocks commitment, and at concentrations of 100 ng/ml or higher the cytokine seems to block mitotic expansion and other steps of differentiation after cell commitment. The identification of a specific time for cell commitment would allow the study of the early genes that might regulate cell reprogramming into adipocytes. (C) 2003 Elsevier Science (USA). All rights reserved.