Effect of Sex Hormones on Crystal Formation in a Stone-forming Rat Model
Effect of Sex Hormones on Crystal Formation in a Stone-forming Rat Model
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DOI：
10.1016/j.urology.2009.09.094
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发表时间：
2010-04-01
期刊：
影响因子：
2.1
通讯作者：
Okuyama, Akihiko
中科院分区：
文献类型：
--
作者：
Yoshioka, Iwao;Tsujihata, Masao;Okuyama, Akihiko
目的 使用形成结石的大鼠模型评估性激素在尿石症中的作用。性激素引起的性别差异被认为影响尿石症的发病率。方法将大鼠分为7组，即完整雄性、睾丸切除雄性、完整雄性皮下植入睾酮、完整雄性皮下植入雌二醇、完整雌性、卵巢切除雌性和完整雌性皮下植入睾酮。 10周龄时，给大鼠喂食含0.5%乙二醇的饮用水，并给予0.5μg 1,25-二羟基维生素D-3。检查各组肾脏晶体沉积和氧化应激程度，并使用实时逆转录聚合酶链反应比较各组之间的内源性草酸代谢和抗氧化酶。结果在完整雄性和给予睾酮的雄性中观察到广泛的晶体沉积，而在完整雌性中发现少量晶体。在切除睾丸的男性和施用雌二醇的受试者中，晶体沉积受到抑制，而在切除卵巢的女性和施用睾酮的女性中，晶体沉积分别略有增强和非常增强。尿草酸排泄的增加与肾晶体沉积平行，睾酮治疗通过增加乙醇酸氧化酶的表达而增强这两者。与没有广泛晶体沉积的组相比，具有广泛晶体沉积的组的氧化应激增加。雌二醇可增强抗氧化酶的表达。 结论 睾酮是肾晶体沉积的促进剂，而雌二醇是肾晶体沉积的抑制剂，可能是因为它们对草酸合成和氧化应激有影响。泌尿学 75: 907-913, 2010。(C) 2010 Elsevier Inc.
OBJECTIVES To evaluate the contribution of sex hormones in urolithiasis using a stone-forming rat model. Gender differences because of sex hormones are thought to influence the incidence of urolithiasis.METHODS We divided rats into 7 groups, such as intact males, orchidectomized males, intact males subcutaneously implanted with testosterone, intact males subcutaneously implanted with estradiol, intact females, ovariectomized females, and intact females subcutaneously implanted with testosterone. At 10 weeks old, the rats were fed 0.5% ethylene glycol in drinking water and given 0.5 mu g of 1,25-dihydroxy vitamin D-3. Kidney crystal deposition and the degree of oxidative stress were examined in each group, and endogenous oxalate metabolism and antioxidant enzymes were compared among groups using real-time reverse transcription-polymerase chain reaction.RESULTS Extensive crystal deposition was observed in intact males and testosterone-administered males, whereas few crystals were found in intact females. Crystal deposition was inhibited in orchiectomized males and in those administered estradiol, whereas ovariectomized females and testosterone-administered females had slightly enhanced and very enhanced crystal deposition, respectively. Increases in urinary oxalate excretion paralleled renal crystal deposition, which were both enhanced by testosterone treatment through increased glycolate oxidase expression. Oxidative stress increased in groups with extensive crystal deposition compared with those without. Antioxidant enzyme expression was enhanced by estradiol.CONCLUSIONS Testosterone was a promoter and estradiol an inhibitor of kidney crystal deposition, likely because of their effects on oxalate synthesis and oxidative stress. UROLOGY 75: 907-913, 2010. (C) 2010 Elsevier Inc.