Conservation and divergence of patterns of expression and lineage-specific transcripts in orthologues and paralogues of the mouse Hox-1.4 gene.
Conservation and divergence of patterns of expression and lineage-specific transcripts in orthologues and paralogues of the mouse Hox-1.4 gene.
复制标题
小鼠 Hox-1.4 基因的直向同源物和旁系同源物中表达模式和谱系特异性转录物的保守性和分歧。
DOI：
10.1006/dbio.1993.1064
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发表时间：
1993
影响因子：
2.7
通讯作者：
Wolgemuth,DJ
中科院分区：
文献类型：
--
作者：
Watrin,F;Wolgemuth,DJ
在确定鼠Hox-1.4基因的直系同源物和旁系同源物表达模式的相似性和差异的研究中，检查了含有同源盒的基因的结构组织与其细胞特异性表达之间可能的相关性。我们首先比较了Hox-1.4亚家族成员、Hox-2.6和Hox-4.2在成年小鼠睾丸中的表达模式。虽然这三个进化相关的基因在胚胎中枢神经系统中表现出相似的前部表达限制，但它们在成年睾丸中表达的细胞特异性却有很大不同。Hox-1.4仅在生殖细胞中大量表达；Hox-2.6在生精细胞和体细胞中均低水平表达； Hox-4.2转录本似乎仅限于体细胞。接下来我们分析了小鼠睾丸中几个Hox-1.4直系同源物的表达，以确定Hox-1簇的其他成员是否参与雄性生殖细胞分化途径。我们发现Hox-1.4的两个相邻基因Hox-1.3和Hox-1.5在种系中表达，但水平较低。此外，与其他成体组织和第 12.5 天胚胎相比，Hox-1.3 和 Hox-1.4 均产生独特的种系特异性转录本。
A possible correlation between the structural organization of homeobox-containing genes and their cell-specific expression was examined in studies determining similarities and differences in the expression patterns of orthologues and paralogues of the murineHox-1.4gene. We first compared the expression pattern of members of theHox-1.4subfamily,Hox-2.6andHox-4.2, in the adult mouse testis. Although these three evolutionarily related genes exhibited similar anterior limits of expression in the embryonic central nervous system, their cellular specificity of expression was very different in the adult testis.Hox-1.4was abundantly expressed only in the germ cells;Hox-2.6was expressed at very low levels in both spermatogenic cells and somatic cells; andHox-4.2transcripts appeared to be restricted to the somatic cells. We next analyzed the expression of several of theHox-1.4orthologues in the mouse testis to determine if other members of theHox-1cluster are involved in the male germ cell differentiation pathway. We showed that the two adjacent genes ofHox-1.4, Hox-1.3andHox-1.5, are expressed in the germ line but at lower levels. Further, bothHox-1.3andHox-1.4produced unique, germ line-specific transcripts as compared to other adult tissues and the Day 12.5 embryo.