Retinal ganglion cell death in experimental glaucoma
Retinal ganglion cell death in experimental glaucoma
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DOI：
10.1136/bjo.84.3.303
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发表时间：
2000-03-01
影响因子：
4.1
通讯作者：
Caprioli, J
中科院分区：
文献类型：
--
作者：
Morgan, JE;Uchida, H;Caprioli, J
AIMS-为了确定是否parasol视网膜神经节细胞（magnocellular途径）是选择性地失去了在灵长类动物模型glaucoma. Methods-眼高血压引起的6只食蟹猴6-14周的一只眼睛。在这些眼睛的视网膜神经节细胞标记逆行示踪辣根过氧化物酶（HRP）植入视神经，随后检查视网膜整体安装准备。视网膜神经节细胞损失的程度估计从尼氏染色的组织相比，与对侧未处理的control eye.Results-In三个hemocomatous视网膜与最好的标记1282个细胞，可以分类，其中182个是parasol细胞和1100个是侏儒细胞。线性回归分析未显示随着细胞丢失增加，阳伞细胞与侏儒细胞的比例显著降低（回归斜率0.023，95%，CI -0.7至0.11）。与对照眼相比，昏迷眼视网膜神经节细胞的索马细胞体缩小了20%（P=0.003），侏儒细胞缩小了16%（P
Aims-To determine whether parasol retinal ganglion cells (magnocellular pathway) are selectively lost in the primate model of glaucoma.Methods-Ocular hypertension was induced in one eye of six Macaca fascicularis monkeys for 6-14 weeks. The retinal ganglion cells in these eyes were labelled retrogradely with the tracer horseradish peroxidase (HRP) implanted into the optic nerve and subsequently examined in retinal whole mount preparations. The degree of retinal ganglion cell loss was estimated from Nissl stained tissue by comparison with the contralateral untreated control eye.Results-In the three glaucomatous retinas with the best labelling 1282 cells could be classified, of which 182 were parasol cells and 1100 were midget cells. Linear regression analysis did not demonstrate a significant reduction in the proportion of parasol to midget cells with increasing cell loss (regression slope 0.023, 95%, CI -0.7 to 0.11). Compared with the control eye the cell soma of the remaining retinal ganglion cells in glaucomatous eyes were reduced in size by 20% for parasol cells (p=0.003) and by 16% for midget cells (p