THE ROLE OF MULTIVALENCY IN ANTIBODY MEDIATED LIPOSOME TARGETING
THE ROLE OF MULTIVALENCY IN ANTIBODY MEDIATED LIPOSOME TARGETING
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DOI：
10.1016/0006-291x(87)91565-8
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发表时间：
1987-06-30
影响因子：
3.1
通讯作者：
HUANG, L
中科院分区：
文献类型：
--
作者：
HOUCK, KS;HUANG, L
我们已经研究了多价性在免疫脂质体结合到细胞中的作用，所述细胞使用具有越来越多的棕榈酰抗H2Kk抗体的脂质体展示不同量的表面抗原，所述棕榈酰抗H2Kk抗体并入到双层中。用蛋白酶k处理RDM-4淋巴瘤细胞以产生一系列具有不同量的H2Kk抗原的细胞。免疫脂质体的结合百分比与RDM-4细胞显示的抗原数量有关。随着每个脂质体中抗体分子数量的增加，观察到脂质体结合增加。然而，与具有较高抗体密度的免疫脂质体相比，具有较低抗体密度的免疫脂质体的高抗原密度细胞与低抗原密度细胞的结合比率较高。该结果表明，为了更好地区分抗原密度不同的细胞，每个脂质体具有较低数量的抗体分子的脂质体可能比具有较大抗体密度的脂质体更有用地作为具有低水平抗原表达的细胞的区分工具。
We have investigated the role of multivalency in immunoliposome binding to cells displaying different amounts of surface antigen using liposomes with increasing numbers of palmitoyl anti-H2Kk antibodies incorporated into the bilayer. RDM-4 lymphoma cells were treated with proteinase k to generate a series of cells with various amounts of H2Kk antigen. Percent binding of immunoliposomes was related to the number of antigens displayed by the RDM-4 cell. Increasing liposome binding was observed with increasing number of antibody molecules per liposome. However, the ratio of binding of the high-antigen-density cells to that of the low-antigen-density cells was higher with immunoliposomes of lower antibody density than the ones with higher antibody density. This result suggests that for better discrimination between cells differing in antigen density, liposomes with lower numbers of antibody molecules per liposome may be more useful as a discriminatory tool for cells with a low level antigen expression than liposomes with greater antibody densities.