Chemical genomic profiling via barcode sequencing to predict compound mode of action.
Chemical genomic profiling via barcode sequencing to predict compound mode of action.
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DOI：
10.1007/978-1-4939-2269-7_23
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
Andersen, Raymond J
中科院分区：
文献类型：
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作者：
Piotrowski, Jeff S;Simpkins, Scott W;Li, Sheena C;Deshpande, Raamesh;McIlwain, Sean J;Ong, Irene M;Myers, Chad L;Boone, Charlie;Andersen, Raymond J
化学基因组学是一种无偏见的全细胞方法，用于表征新化合物以确定作用模式和细胞靶点。我们的这种技术是建立在酿酒酵母的条形码缺失突变体上的，并且已经适应了使用下一代测序的高通量方法。在这里，我们描述了从感兴趣的化合物生成化学基因组图谱的步骤，以及如何使用这些信息来预测生物活性化合物的分子机制和靶点。
Chemical genomics is an unbiased, whole-cell approach to characterizing novel compounds to determine mode of action and cellular target. Our version of this technique is built upon barcoded deletion mutants of Saccharomyces cerevisiae and has been adapted to a high-throughput methodology using next-generation sequencing. Here we describe the steps to generate a chemical genomic profile from a compound of interest, and how to use this information to predict molecular mechanism and targets of bioactive compounds.