An Integrated Resource for Barley Linkage Map and Malting Quality QTL Alignment
An Integrated Resource for Barley Linkage Map and Malting Quality QTL Alignment
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DOI：
10.3835/plantgenome2008.01.0005
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发表时间：
2009-07
期刊：
影响因子：
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通讯作者：
P. Szűcs;V. C. Blake;Prasanna Bhat;S. Chao;T. Close;A. Cuesta‐Marcos;G. Muehlbauer;L. Ramsay;R. Waugh;P. Hayes
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文献类型：
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作者：
P. Szűcs;V. C. Blake;Prasanna Bhat;S. Chao;T. Close;A. Cuesta‐Marcos;G. Muehlbauer;L. Ramsay;R. Waugh;P. Hayes
大麦（Hordeum vulgare L.）是遗传学研究的重要经济模式植物。大麦目前正通过一套越来越全面的遗传分析工具来服务，这些工具最近通过基于基因的单核苷酸多态性（SNP）构建的高密度遗传连锁图谱得到了增强。这些基于SNP的图谱需要通过将多种类型的标记整合到单个图谱中来与用于数量性状基因座（QTL）检测的早期图谱进行比对。使用俄勒冈州沃尔夫大麦（OWB）作图群体开发了2383位点连锁图以允许这样的比对。该图谱基于1472个SNP，722个DArT和189个先前标记，包括形态学，简单序列重复（SSR），限制性片段长度多态性（RFLP）和序列标记位点（STS）位点。因此，这种新的OWB图谱形成了高密度SNP图谱和先前QTL报告之间的有用桥梁。如文献中所报道的，使用来自多个作图群体的麦芽质量QTL显示了这种桥梁概念的应用。这是在GrainGenes网站上为所有类型的QTL和地图开发大麦QTL社区管理工作手册的第一步。OWB相关资源可在OWB Data and GrainGenes Tools（OWB‐DGGT）（http：//wheat.tag.usda.gov/ggpages/maps/OWB/）获得。
Barley (Hordeum vulgare L.) is an economically important model plant for genetics research. Barley is currently served by an increasingly comprehensive set of tools for genetic analysis that have recently been augmented by high‐density genetic linkage maps built with gene‐based single nucleotide polymorphisms (SNPs). These SNP‐based maps need to be aligned with earlier generation maps, which were used for quantitative trait locus (QTL) detection, by integrating multiple types of markers into a single map. A 2383 locus linkage map was developed using the Oregon Wolfe Barley (OWB) Mapping Population to allow such alignments. The map is based on 1472 SNP, 722 DArT, and 189 prior markers which include morphological, simple sequence repeat (SSR), Restriction Fragment Length Polymorphism (RFLP), and sequence tagged site (STS) loci. This new OWB map forms, therefore, a useful bridge between high‐density SNP‐only maps and prior QTL reports. The application of this bridge concept is shown using malting‐quality QTLs from multiple mapping populations, as reported in the literature. This is the first step toward developing a Barley QTL Community Curation workbook for all types of QTLs and maps, on the GrainGenes website. The OWB‐related resources are available at OWB Data and GrainGenes Tools (OWB‐DGGT) (http://wheat.pw.usda.gov/ggpages/maps/OWB/).