PIEZO2: A Novel Molecule Involved in the Development of AIS
PIEZO2: A Novel Molecule Involved in the Development of AIS
复制标题
PIEZO2：一种参与 AIS 开发的新型分子。
DOI：
10.1097/brs.0000000000003224
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发表时间：
2020-02-01
期刊：
影响因子：
3
通讯作者：
Zhu, Zezhang
中科院分区：
文献类型：
--
作者：
Wu, Zhichong;Wang, Yuwen;Zhu, Zezhang
研究设计。病例对照研究。目标。本研究旨在探讨PIEZO2基因在AIS发病中的潜在作用。背景数据摘要。据报道，PIEZO2基因突变与进行性脊柱侧弯和本体感觉受损有关。先前的研究表明，AIS患者的本体感觉可能受损。然而，对于AIS患者本体感觉的机制以及PIEZO2基因在AIS病因学中的作用尚缺乏了解。方法：研究方法。对AIS患者和年龄匹配的健康对照组进行本体感觉测试。根据下降的风险评分，将AIS患者分为本体感觉受损组和本体感觉未受损组。术中采集34例AIS患者的椎旁肌。比较损伤组和非损伤组Piezo2的组织表达。此外，还比较了两组肌梭内肌纤维的平均数量。结果。本体感觉测试显示患者的跌倒指数显著高于对照组(41.70±16.5vs.11.3+/-8.3，P=0.004)。损伤组Piezo2基因表达显著降低(0.51±0.24vs.1.00+/-0.33，P=0.04)。AIS受损组肌梭内平均肌纤维数量较非受损组显著减少(2.2±1.3比3.5+/-2.1，P=0.04)。Piezo2的表达水平与肌梭内肌纤维的平均数量呈显著正相关(r=0.352.0 5，P=0.0 4)。结论。AIS患者本体感觉明显受损。Piezo2的异常表达可能通过改变本体感觉和肌梭中的肌纤维数量而在AIS中发挥作用。AIS中Piezo2基因表达的调控机制有待进一步研究。
Study Design. A case-control study. Objective. This study aimed to investigate the potential role ofPIEZO2gene in the development of AIS. Summary of Background Data. Mutations ofPIEZO2gene have been reported to be associated with progressive scoliosis and impaired proprioception. Previous studies showed that patients with AIS may have impaired proprioception. However, there is lack of knowledge concerning the mechanism underlying the proprioception of AIS patients and the role ofPIEZO2gene in the etiology of AIS. Methods. Proprioception tests were performed in both AIS patients and age-matched healthy controls. Based on the falling risk scores, AIS patients were divided into impaired proprioception group and unimpaired proprioception group. Paraspinal muscle was collected from 34 AIS patients during surgery. The tissue expression of PIEZO2 was compared between the impaired group and the unimpaired group. In addition, the average number of muscle fibers in the muscle spindle was compared between the two groups. Results. Proprioception test showed that patients had significantly higher falling index (41.7 +/- 16.5vs.11.3 +/- 8.3,P = 0.004). In addition, the expression of PIEZO2 gene was remarkably decreased in the impaired group (0.51 +/- 0.24vs.1.00 +/- 0.33,P = 0.04). The average number of muscle fibers in the muscle spindle was significantly decreased in AIS patients of the impaired group than those of the unimpaired group (2.2 +/- 1.3vs.3.5 +/- 2.1,P = 0.04). PIEZO2 expression level was remarkably correlated with the average number of muscle fibers in the muscle spindle (r = 0.352,P = 0.04). Conclusion. Proprioception is remarkably impaired in patients with AIS. Abnormal expression of PIEZO2 may play a role in AIS via altered proprioception and number of muscle fibers in the muscle spindles. Further investigation is warranted to illustrate the mechanism regulating PIEZO2 expression in AIS.