Culture of chicken embryos in surrogate eggshells
Culture of chicken embryos in surrogate eggshells
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DOI：
10.1093/ps/84.9.1477
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发表时间：
2005-09-01
期刊：
影响因子：
4.4
通讯作者：
Petitte, JN
中科院分区：
文献类型：
--
作者：
Borwornpinyo, S;Brake, J;Petitte, JN
鸡胚是研究胚胎发育的经典模型。然而，大多数研究者还没有研究胚胎操作对鸡孵化率的影响。本研究的目的是确定鸡蛋的方向和密封膜的类型对培养胚胎的孵化率的影响。在受体代孕鸡蛋壳的小端制作窗口，并将供体胚胎放入受体蛋壳中进行前3d的孵育。当用萨兰包装密封的开窗鸡蛋放置时，与窗口端朝上相比，窗口端朝下放置时，前3d的存活率最高（P < 0.05）。将三粘土龄的培养胚胎转移到受体火鸡蛋壳中，用保鲜膜密封，并培养至孵化。水的重量损失的替代蛋壳文化，无论保鲜膜类型是没有显着不同的控制完整的鸡蛋。在火鸡蛋壳中培养并用Handi Wrap密封的胚胎表现出比用萨兰Wrap密封的培养物（45.2%+/- 13.8%）更高的孵化率（75%+/- 10.2%）。对照完整蛋的孵化率（86.4% ± 5.3%）与用Handi Wrap密封的蛋的孵化率差异不显著（P > 0.05），表明Handi Wrap是孵化3d后培养的鸡胚的良好密封剂。
The chick embryo is a classical model to study embryonic development. However, most researchers have not studied the effect of embryonic manipulation on chick hatchability. The objective of this study was to determine the effect of egg orientation and type of sealing film on the hatchability of cultured embryos. Windows were made in the small end of recipient surrogate chicken eggshells, and donor embryos were placed into the recipient eggshell for the first 3 d of incubation. Survival over the first 3 d was maximized (P < 0.05) when windowed eggs sealed with Saran Wrap were positioned with the window-end down compared with window-end up. Three-clay-old cultured embryos were transferred into recipient turkey eggshells, sealed with cling film, and cultured until hatch. Water weight loss of the surrogate eggshell cultures regardless of cling film type was not significantly different from control intact eggs. The embryos cultured in turkey eggshells and sealed with Handi Wrap exhibited higher hatchability (75% +/- 10.2%) than cultures sealed with Saran Wrap (45.2% +/- 13.8%). Hatchability of control intact eggs (86.4% +/- 5.3%) was not significantly (P > 0.05) different from the hatchability of eggs sealed with Handi Wrap, which suggested that Handi Wrap was an excellent sealant for chick embryos cultured after 3 d of incubation.